Methods and compositions to prevent formation of adhesions in biological tissues

• US 7,029,688 B2
• Filed: 03/28/2003
• Issued: 04/18/2006
• Est. Priority Date: 08/27/1997
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Hubbell, Jeffrey A., Elbert, Donald L., Winblade, Natalie D.
• Primary Examiner(s): Webman, Edward J.
• Application Number:
  US10/403,344
• Publication Number:
  US 20030185787A1
• Time in Patent Office: 1,117 Days
• Field of Search: 424422- 23, 514/772.1, 427/2.12, 427/2.24, 427/2.28
• US Class Current: 424/422
• CPC Class Codes: A61K 31/77 of oxiranes
• CPC Class Codes: A61K 31/78 of acrylic acid or derivatives thereof
• CPC Class Codes: A61K 31/79 Polymers of vinyl pyrrolidone
• CPC Class Codes: A61K 31/80 Polymers containing hetero atoms not provided for in groups A61K31/755 - A61K31/795
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 31/06 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds A61L31/041 takes precedence
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: C08L 101/02 characterised by the presence of specified groups , e.g. terminal or pendant functional groups
• CPC Class Codes: C08L 71/02 Polyalkylene oxides
• CPC Class Codes: Y10S 525/937 Utility as body contact e.g. implant, contact lens or I.U.D.
• CPC Class Codes: Y10S 977/931 Medical device coating

Abstract

The invention discloses materials that adsorb readily to the surfaces of body tissues in situ and provide a steric barrier between such tissues, so that tissue adhesions, which typically form following surgical procedures, are minimized. These materials contain a polymer of hydrophilic molecules such as polyethylene glycol (PEG) bound to a polymer that spontaneously adsorbs to biological tissue such as phenylboronic acid (PBA). The PEG-PBA co-polymer can be formed in a variety of geometries. The materials can also be used to coat prosthetics and other implants.

Claims

1. A method for altering cell or tissue interaction with a surface comprising applying to the surface an effective amount of a copolymer comprising an adsorbing segment containing boronate groups and an adhesion resistant segment,

wherein the copolymer is selected from the group consisting of block copolymers where both segments are linear, dendritic copolymers where the adsorbing segment is dendritic and brush copolymers where the adsorbing segment is the copolymer backbone,

wherein the adhesion resistant segment is effective to prevent cellular attachment or deposition of biological material on a surface coated with the copolymer.

2. The method of claim 1, wherein the copolymer is applied in an amount effective to minimize or prevent tissue adhesion.

3. The method of claim 1, wherein the copolymer is applied in an amount effective to decrease the risk of thrombosis, decrease inflammation, or decrease the abnormal proliferation of cells.

4. The method of claim 1, wherein the copolymer is applied in an amount effective to enhance wound healing.

5. The method of claim 1, wherein the copolymer is applied in an amount effective for the delivery of a bioactive, prophylactic, or diagnostic agent.

6. The method of claim 1, wherein the surface is the surface of a prosthesis or other implantable device.

7. The method of claim 1, wherein the surface is a surface of a cell or tissue.

8. The method of claim 1, wherein the surface is a region where tissue has been injured.

9. The method of claim 1, wherein the copolymer is applied at the time of or shortly after tissue injury.

10. The method of claim 1, wherein the copolymer is applied using a catheter or other minimally invasive technique.

11. The method of claim 1, wherein the adsorbing segment comprises a boronate group selected from the group consisting of phenylboronic acid (PBA), 2-carboxyethaneboronic acid, 1,2-dicarboxyethaneboronic acid, β

,β

′

-dicarboxyethaneboronate, β

,γ

-dicarboxypropaneboronate, 2-nitro- and 4-nitro-3-succinamidobenzene boronic acids, 3-nitro-4-(6-aminohexylamido)phenyl boronic acid, {4-[(hexamethylenetetramine)methyl]phenyl}boronic acid, 4-(N-methyl)carboxamidobenzene boronic acid, 2-{[(4-boronphenyl)methyl]-ethylammonio}ethyl and 2-{[(4-boronphenyl)methyl]diethylammonio}-ethyl groups, succinyl-3-aminophenylboronic add, 6-aminocaproyl-3-aminophenylboronic acid, 3-(N-succinimidoxycarbonyl)aminophenylboronate, p-(ω

-aminoethyl)phenylboronate, p-vinylbenzeneboronate, N-(3-dihydroxyborylphenyl)succinamic acid, N-(4-nitro-3-dihydroxyborylphenyl)succinamic acid, O-dimethylaminomethylbenzeneboronic acid, 4-carboxybenzeneboronic acid, 4-(N-octyl)carboxamidobenzeneboronic acid, 3-nitro-4-carboxybenzeneboronic acid, 2-nitro-4-carboxybenzeneboronic acid, 4-bromophenylboronate, p-vinylbenzene boronate, 4-(ω

-aminoethyl)phenylboronate, catechol [2-(diethylamino)carbonyl, 4-bromomethyl]phenyl boronate, and 5-vinyl-2-dimethylaminomethylbenzeneboronic acid.

12. The method of claim 1, wherein the adsorbing segment comprises phenylboronic acid.

13. The method of claim 1, wherein the adhesion resistant segment is selected from the group consisting of polyalkylene oxides, mixed polyalkylene oxides having a solubility of at least one gram/liter in aqueous solutions, water soluble polysaccharides, polyvinyl alcohol, poly-N-vinyl pyrrolidone, noncationic polyacrylates, noncationic polymethacrylates, and mixtures and copolymers thereof.

14. The method of claim 1, wherein the adhesion resistant segment is polyethylene glycol.

15. The method of claim 1, wherein the copolymer is a block copolymer.

16. The method of claim 1, wherein the copolymer is a brush copolymer.

17. The method of claim 1, wherein the copolymer is a dendritic copolymer.

18. The method of claim 1, further comprising a pharmaceutically acceptable carrier.

19. The method of claim 1, further comprising a biodegradable linkage.

20. The method of claim 1, wherein the copolymer further comprises a bioactive, therapeutic, or diagnostic agent.

21. The method of claim 20, wherein the agent is chemically coupled to the copolymer.

22. The method of claim 1, wherein the adsorbing segment comprises electron withdrawing groups so that the effective pKa of the copolymer is lower than that of the copolymer without electron withdrawing groups.

23. The method of claim 1, wherein the adsorbing segment comprises amine groups so that the effective pKa of the copolymer is lower than that of the copolymer without amine groups.