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Method for differentiating rat hepatic stem cells to insulin-producing cells

  • US 7,029,915 B2
  • Filed: 02/24/2003
  • Issued: 04/18/2006
  • Est. Priority Date: 02/22/2002
  • Status: Expired due to Fees
First Claim
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1. A method for making cells having a pancreatic endocrine cell phenotype, the method comprising the steps of:

  • (a) isolating hepatic stem cells from the livers of 2-acetylamino-fluorene-treated adult rats; and

    (b) culturing said hepatic stem cells in vitro in a medium comprising greater than 5.5 mM glucose until said hepatic stem cells differentiate into cells having a pancreatic endocrine cell phenotype.

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