Thermocycler

• US 7,030,340 B2
• Filed: 06/02/2004
• Issued: 04/18/2006
• Est. Priority Date: 06/04/2003
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Siemens AG
• Inventors: Knoche, Jochem
• Primary Examiner(s): PELHAM, JOSEPH MOORE
• Application Number:
  US10/859,420
• Publication Number:
  US 20040265884A1
• Time in Patent Office: 685 Days
• Field of Search: None
• US Class Current: 219/385
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 2300/1844 using fans
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/5255 by moving sample containers
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]

Abstract

A thermocycler has, as heating devices, a plurality of heating blocks each with a respective coupling face, for heating at least one stationary specimen that during one temperature cycle has a plurality of temperature levels. The plurality of heating blocks is pivotably disposed, and the pivot axis is oriented perpendicularly to the coupling faces. The thermocycler is especially well suited for performing the polymerase chain reaction.

Claims

1. A thermocycler having a plurality of heating devices for heating at least one stationary specimen that during one temperature cycle has a plurality of temperature levels, the plurality of heating devices comprising;

movable heating blocks, each of the plurality of heating blocks provided with a coupling face for heating the stationary specimen,

wherein each of the plurality of movable heating blocks is pivotably disposed about a pivot axis, the pivot axis being oriented perpendicularly to the coupling faces of the plurality of heating blocks, and

wherein the heating blocks are displaceable perpendicularly to a respective coupling face via a hoisting device, the hoisting device comprises a deflection lever.

2. The thermocycler of claim 1, wherein the heating blocks are retained in a pivotable support plate.

3. The thermocycler of claim 1, wherein the heating blocks are surrounded by an insulating sleeve outside the respective coupling faces.

4. The thermocycler of claim 1, wherein the coupling face of each of the plurality of heating blocks has an area of at least 100 mm².

5. The thermocycler of claim 1, further comprising a fan that cools the plurality of heating blocks outside of their respective coupling faces.

6. The thermocycler of claim 1, wherein each of the plurality of the heating blocks has a heating element.

7. The thermocycler of claim 6, wherein the heating element is a resistance heating element.

8. The thermocycler of claim 6, wherein the heating element is a power transistor.

9. The thermocycler of claim 1, wherein each of the plurality of heating blocks has a temperature sensor.

10. The thermocycler of claim 8, wherein the power transistor is provided as a temperature sensor.

11. The thermocycler of claim 8 further comprising a temperature regulating device.

12. The thermocycler of claim 1 further comprising a position control device.

13. The thermocycler of claim 1 further comprises a system controller operably cooperating with a temperature regulating device and a position control device.

14. The thermocycler of claim 1 further comprising a system controller linking a position control unit and temperature regulating device, such that a selection of a heating block is communicated to the position control device.

15. The thermocycler of claim 14, wherein the temperature regulating device includes a controller unit, which receives set-point temperature values specified by the system controller for a temperature cycle to be communicated to heating elements of each of the plurality heating blocks and reports temperature values collected by temperature sensors associated with each of the plurality heating blocks back to the system controller, the controller unit having a memory operably connected to the controller unit and a software stored in the memory.

16. The thermocycler of claim 15, wherein the temperature sensors and heating elements of each of the heating blocks are connected to the controller unit, the controller unit setting a temperature difference between the specimen to be heated by one of the plurality of heating blocks and the corresponding heating block before the specimen is put into contact with the coupling face of the corresponding heating block different than a temperature change in the specimen to be effected.

17. A thermocycler having a plurality of heating devices for heating at least one stationary specimen that during one temperature cycle has a plurality of temperature levels, the plurality of heating devices comprising:

movable heating blocks, each of the plurality of heating blocks provided with a coupling face for heating the stationary specimen,

wherein each of the plurality of movable heating blocks is pivotably disposed about a pivot axis, the pivot axis being oriented perpendicularly to the coupling faces of the plurality of heating blocks, and

wherein the heating blocks are displaceable perpendicularly to a respective coupling face via a hoisting device, the hoisting device comprises a tension magnet.

18. A method for heating a specimen in a thermocycler, having a plurality of heating blocks for heating at least one specimen that during one temperature cycle has a plurality of temperature levels, the method comprising:

moving the plurality of heating blocks, each of the plurality of heating blocks provided with a coupling face for heating the specimen; and

setting a temperature difference between the specimen to be heated by one of the plurality of heating blocks and the corresponding heating block before the specimen is put into contact with the coupling face of the corresponding heating block different than a temperature change in the specimen to be effected.

19. The method of claim 18, wherein setting the temperature comprises setting the temperature difference between the specimen to be heated by the one of the plurality of heating blocks and the corresponding heating block before the specimen is put into contact with the coupling face of the corresponding heating block higher than a temperature change in the specimen to be effected.