Assays for amphetamine and methamphetamine using stereospecific reagents
First Claim
Patent Images
1. A compound of the formula:
-
wherein;
R1 is H, lower alkyl, a protecting group,R2 is H, lower alkyl, a protecting group,L is —
(CH2)t—
X—
(CH2)v—
Y—
wherein X is C(O) or SO2, Y is a bond, S or —
NR3 wherein R3 is H or lower alkyl, W is O, S, or NH, and t is an integer from 1 to 6 and v is an integer from 2 to 6,Z is H, a protecting group, a poly(amino acid), a non-poly(amino acid) label moiety, a non-poly(amino acid) immunogenic carrier, or a functional group excluding thiol,n is 1 when Z is other than a poly(amino acid) or, when Z is a poly(amino acid), n is an integer between 1 and the molecular weight of the poly(amino acid) divided by 500;
and salts thereof.
4 Assignments
0 Petitions
Accused Products
Abstract
Methods, compositions and kits are disclosed. Enzyme conjugates of Formula I may be employed in assays for the determination of an amphetamine and/or a methamphetamine. Immunogenic conjugates of Formula I may be employed to prepare antibodies for an amphetamine and/or for a methamphetamine for use in assays for the determination of an amphetamine and/or a methamphetamine. The enzyme conjugates may also be employed to screen antibodies for use in such methods.
-
Citations
59 Claims
-
1. A compound of the formula:
-
wherein; R1 is H, lower alkyl, a protecting group, R2 is H, lower alkyl, a protecting group, L is —
(CH2)t—
X—
(CH2)v—
Y—
wherein X is C(O) or SO2, Y is a bond, S or —
NR3 wherein R3 is H or lower alkyl, W is O, S, or NH, and t is an integer from 1 to 6 and v is an integer from 2 to 6,Z is H, a protecting group, a poly(amino acid), a non-poly(amino acid) label moiety, a non-poly(amino acid) immunogenic carrier, or a functional group excluding thiol, n is 1 when Z is other than a poly(amino acid) or, when Z is a poly(amino acid), n is an integer between 1 and the molecular weight of the poly(amino acid) divided by 500; and salts thereof. - View Dependent Claims (2, 3, 4, 5, 6)
-
-
7. A compound of the formula:
-
wherein; R1′
is H, lower alkyl, a protecting group,R2′
is H, alkyl, a protecting group,W′
is O, S or NR3 wherein R3 is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3 wherein R3 is H or lower alkyl,Z′
is H, a protecting group, a poly(amino acid), a non-poly(amino acid) label moiety, a non-poly(amino acid) immunogenic carrier, or a functional group,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is 1 when Z′
is other than a poly(amino acid) or, when Z′
is a poly(amino acid), n′
is an integer between 1 and the molecular weight of the poly(amino acid) divided by 500;and salts thereof. - View Dependent Claims (8, 9, 10, 11, 12)
-
-
13. A compound of the formula:
-
wherein; R1″
is H, lower alkyl, a protecting group,R2″
is H, lower alkyl, a protecting group,W″
is O, S, or NR3′
wherein R3′
is H or lower alkyl,Y″
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z″
is H, a protecting group, a poly(amino acid), a non-poly(amino acid) label moiety, a non-poly(amino acid) immunogenic carrier, or a functional group,t″
is an integer from 1 to 6 and v″
is an integer from 2 to 6,n″
is 1 when Z″
is other than a poly(amino acid) or, when Z″
is a poly(amino acid), n″
is an integer between 1 and the molecular weight of the poly(amino acid) divided by 500;and salts thereof. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 31, 32, 33, 34, 35, 36, 37, 38)
-
-
22. A compound of the formula:
-
wherein; R1′
″
is H, lower alkyl, a protecting group,R2′
″
is H, lower alkyl, a protecting group,W″
is O, S, or NR3′
″
wherein R3′
″
is H or lower alkyl,Y′
″
is a bond, S or —
NR3′
″
wherein R3′
″
is H or lower alkyl,Z′
″
is H, a protecting group, a poly(amino acid), a non-poly(amino acid) label moiety, a non-poly(amino acid) immunogenic carrier, or a functional group,t′
″
is an integer from 1 to 6 and v′
″
is an integer from 2 to 6,n′
″
is 1 when Z′
″
is other than a poly(amino acid) or, when Z′
″
is a poly(amino acid), n′
″
is an integer between 1 and the molecular weight of the poly(amino acid) divided by 500;and salts thereof. - View Dependent Claims (23, 24, 25, 26, 27, 28, 29, 30, 39, 40, 41, 42, 43, 44, 45, 46)
-
-
47. A method for determining amphetamine and/or methamphetamine in a sample suspected of containing amphetamine and/or methamphetamine, said method comprising:
-
(a) providing in combination in a medium; (i) said sample, (ii) an antibody for amphetamine, (iii) an antibody for methamphetamine, (iv) a compound of the formula;
wherein; R1′
is H, lower alkyl, a protecting group,R2′
is H, lower alkyl, a protecting group,W′
is O, S, or NR3′
wherein R3′
is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z′
is an enzyme,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is an integer between 1 and the molecular weight of said enzyme divided by 500; and(b) examining said medium for the presence of a complex comprising said amphetamine and said antibody for amphetamine and/or a complex of said methamphetamine and said antibody for methamphetamine, the presence thereof indicating the presence of said amphetamine and/or methamphetamine in said sample. - View Dependent Claims (48, 49, 50, 51)
-
-
52. A method for determining amphetamine and/or methamphetamine in a sample suspected of containing amphetamine and/or methamphetamine, said method comprising:
-
(a) providing in combination in a medium; (i) said sample, (ii) a conjugate of an enzyme and an amphetamine analog and/or a conjugate of an enzyme and a methamphetamine analog, (iii) an antibody for amphetamine, said antibody being raised against a compound of the formula;
wherein; R1′
is H and R2′
is H,W′
is O, S, or NR3′
wherein R3′
is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z′
is a protein immunogenic carrier or a non-poly(amino acid) immunogenic carrier,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is an integer between 1 and the molecular weight of said antigen or said immunogenic carrier divided by 500; and
/or(iv) an antibody for methamphetamine, said antibody being raised against a compound of the formula;
wherein; R1′
is H and R2′
is methyl,W′
is O, S, or NR3′
wherein R3′
is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z′
is a protein immunogenic carrier or a non-poly(amino acid) immunogenic carrier,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is an integer between 1 and the molecular weight of said antigen or said immunogenic carrier divided by 500; and(b) examining said medium for the presence of a complex comprising said amphetamine and said antibody for amphetamine and/or a complex of said methamphetamine and said antibody for methamphetamine, the presence thereof indicating the presence of said amphetamine and/or methamphetamine in said sample. - View Dependent Claims (53, 54, 55, 56)
-
-
57. A kit comprising in packaged combination:
-
(i) an antibody for amphetamine, (ii) an antibody for methamphetamine, (iii) a compound of the formula;
wherein; R1′
is H, lower alkyl, a protecting group,R2′
is H, lower alkyl, a protecting group,W′
is O, S, or NR3′
wherein R3′
is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z′
is an enzyme,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is an integer between 1 and the molecular weight of said enzyme divided by 500.- View Dependent Claims (58)
-
-
59. A kit comprising in packaged combination:
-
(i) a conjugate of an enzyme and an amphetamine analog and/or a conjugate of an enzyme and a methamphetamine analog, (ii) an antibody for amphetamine, said antibody being raised against a compound of the formula;
wherein; R1′
is H and R2′
is H,W′
is O, S, or NR3′
wherein R3′
is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z′
is a protein immunogenic carrier or a non-poly(amino acid) immunogenic carrier,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is an integer between 1 and the molecular weight of said antigen or said immunogenic carrier divided by 500; and
/or(iii) an antibody for methamphetamine, said antibody being raised against a compound of the formula;
wherein; R1′
is H and R2′
is methyl,W″
is O, S, or NR3′
wherein R3′
is H or lower alkyl,X′
is C(O) or SO2,Y′
is bond, S or —
NR3′
wherein R3′
is H or lower alkyl,Z′
is a protein immunogenic carrier or a non-poly(amino acid) immunogenic carrier,t′
is an integer from 1 to 6 and v′
is an integer from 2 to 6,n′
is an integer between 1 and the molecular weight of said antigen or said immunogenic carrier divided by 500.
-
Specification