Micro-channel design features that facilitate centripetal fluid transfer

• US 7,041,258 B2
• Filed: 01/03/2003
• Issued: 05/09/2006
• Est. Priority Date: 07/26/2002
• Status: Active Grant

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Shigeura, John, Desmond, Sean M.
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US10/336,330
• Publication Number:
  US 20040018117A1
• Time in Patent Office: 1,222 Days
• Field of Search: 422/72, 422100-102, 436/45, 436/177, 435/288.2, 435/288.4, 435/288.5
• US Class Current: 422/503
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features that facilitate centripetal fluid transfer are also provided.

Claims

1. A sample processing system comprising:

a microfluidic device wherein the device has a length and a width, the microfluidic device comprising a substrate having a first surface, an opposite second surface, and a thickness, an input port formed in at least one of the first surface or a second surface, a manifold formed in the substrate and in fluid communication with the input port, the manifold including a feed channel that extends in a first direction, and a plurality of branch channels each branching off the feed channel and each terminating at a closed end, and a plurality of closed chambers formed in the substrate, at least one of the closed chambers adjacent a respective closed end of the plurality of branch channels; and

a processing apparatus, the processing apparatus comprising

a rotatable platen having an axis of rotation,

a holder capable of holding the microfluidic device on or in the platen, and being disposed off-center with respect to the axis of rotation, and

a drive unit for rotating the platen about the axis of rotation,

wherein the microfluidic device is held by the holder such that a radius of the platen which is parallel to the length or the width of the microfluidic device does not intercept the other of the width or the length, respectively, of the microfluidic device.

2. A sample processing system comprising a microfluidic device and a processing apparatus, the microfluidic device comprising:

a substrate having a first surface, an opposite second surface, and a thickness,

an input port formed in at least one of the first and second surfaces, and

a manifold formed in the substrate and in fluid communication with the input port, the manifold including a feed channel that extends in a first direction, and

a plurality of branch channels branching off the feed channel; and

the processing apparatus comprising;

a rotatable platen having an axis of rotation,

a holder capable of holding a microfluidic device on or in the platen and capable of holding the microfluidic device off-center with respect to the axis of rotation; and

a drive unit to rotate the platen about the axis of rotation.

3. The sample processing system of claim 2, wherein the microfluidic device comprises a plurality of input ports formed in the substrate, and a respective plurality of manifolds in fluid communication with the plurality of input ports.

4. The sample processing system of claim 2, wherein the microfluidic device is held by the holder on the platen, and the input port is radially closer than the plurality of branch channels to the axis of rotation.

5. The sample processing system of claim 2, further including a drive control unit for controlling the drive unit.

6. The sample processing system of claim 2, wherein the plurality of branch channels are parallel to one another.