Electronic transfer moieties attached to peptide nucleic acids

• US 7,045,285 B1
• Filed: 04/21/2000
• Issued: 05/16/2006
• Est. Priority Date: 11/05/1996
• Status: Expired due to Fees

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: O'Connor, Stephen D., Meade, Thomas J., Yu, Changjun, Kayyem, Jon F., Gozin, Michael
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US09/557,577
• Time in Patent Office: 2,216 Days
• Field of Search: 435/4, 435/6, 435/7.1, 422/50, 422/68.1, 436/501, 536/23.1, 536/24.3
• US Class Current: 435/6.11
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors

Abstract

The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.

Claims

1. A peptide nucleic acid comprising at least one monomeric subunit having the formula:

3. A composition according to claim 1 or 2 wherein said monomeric subunit is an internal subunit.

4. A composition according to claim 1 or 2 wherein said electron transfer moiety is an electrode and said linker is a conductive oligomer.

5. A composition according to claim 1 or 2 wherein said electron transfer moiety is a transition metal complex.

6. A composition according to claim 5 wherein said transition metal complex is a metallocene.

7. A composition according to claim 6 wherein said metallocene is a ferrocene.

2. A peptide nucleic acid comprising at least one monomeric subunit having the formula: