Detection of RNA Sequences
First Claim
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1. A method for detecting the presence of a target nucleic acid comprising:
- a) cleaving an invasive cleavage structure, said invasive cleavage structure comprising an RNA target nucleic acid; and
b) detecting the cleavage of said invasive cleavage structure.
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Abstract
The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5′ nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
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39 Claims
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1. A method for detecting the presence of a target nucleic acid comprising:
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a) cleaving an invasive cleavage structure, said invasive cleavage structure comprising an RNA target nucleic acid; and
b) detecting the cleavage of said invasive cleavage structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification