Methods and systems for in situ calibration of imaging in biological analysis
First Claim
Patent Images
1. A method for determining operating characteristics of a photometric detector comprising a plurality of photosensitive pixels, the method comprising:
- illuminating an active surface of the photometric detector with electromagnetic radiation;
recording a plurality of varying brightness readings for the various pixels in the plurality of photosensitive pixels on the photometric detector;
calculating signal statistical dispersion values for the plurality of photosensitive pixels independently on a pixel-by-pixel basis over the plurality of varying brightness readings;
calculating stable intensity values for the plurality of photosensitive pixels independently on a pixel-by-pixel basis over the plurality of varying brightness readings; and
approximating a photon transfer curve based on the signal statistical dispersion values and the stable intensity valueswherein the photometric detector is adapted for biological analysis.
6 Assignments
0 Petitions
Accused Products
Abstract
Software, methods, and systems for calibrating photometric devices are provided. These involve using a non-uniform test illumination field to approximate a photon transfer curve by calculating stable pixel values and statistical dispersions on a pixel-by-pixel basis.
13 Citations
32 Claims
-
1. A method for determining operating characteristics of a photometric detector comprising a plurality of photosensitive pixels, the method comprising:
-
illuminating an active surface of the photometric detector with electromagnetic radiation; recording a plurality of varying brightness readings for the various pixels in the plurality of photosensitive pixels on the photometric detector; calculating signal statistical dispersion values for the plurality of photosensitive pixels independently on a pixel-by-pixel basis over the plurality of varying brightness readings; calculating stable intensity values for the plurality of photosensitive pixels independently on a pixel-by-pixel basis over the plurality of varying brightness readings; and approximating a photon transfer curve based on the signal statistical dispersion values and the stable intensity values wherein the photometric detector is adapted for biological analysis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
-
-
17. A method for determining operating characteristics of a photometric detector, in situ, within an instrument, the photometric detector comprising a plurality of photosensitive pixels, the method comprising:
-
illuminating an active surface of the photometric detector with electromagnetic radiation, wherein the electromagnetic radiation is spatially non-uniform in intensity; recording a plurality of varying brightness readings for the various pixels in the plurality of photosensitive pixels on the photometric detector; calculating signal statistical dispersion values for the plurality of photosensitive pixels independently on a pixel-by-pixel basis over the plurality of varying brightness readings; calculating stable intensity values for the plurality of photosensitive pixels independently on a pixel-by-pixel basis over the plurality of varying brightness readings; approximating a photon transfer curve based on the signal statistical dispersion values and the stable intensity values; and providing a proper operation indication regarding the photometric detector based on the photon transfer curve to indicate whether the photometric detector is operating properly, wherein the instrument is adapted to imaging for biological analysis.
-
-
18. Apparatus for determining operating characteristics of a photometric sensing device contained within an instrument, the apparatus comprising:
-
a photometric sensor comprising a plurality of photosites capable of sensing intensity of electromagnetic energy at the plurality of photosites; a test illumination field generator for generating a test illumination field optically coupled to the photometric sensor, the test illumination field comprising regions presenting a range of intensities to the photometric sensor; a photometric sensor interface circuit coupled to the photometric sensor, the photometric sensor interface circuit operable to read out sets of photosite intensity values from the plurality of photosites, the sets of photosite intensity values corresponding to the intensity of electromagnetic energy sensed at the plurality of photosites; and a memory coupled to the photometric sensor interface circuit, the memory comprising machine readable instructions comprising; read instructions for reading a plurality of varying brightness readings of the sets of photosite intensity values while exposed to the test illumination field; noise determination instructions for calculating statistical dispersions of the sets of photosite intensity values corresponding to the plurality of varying brightness readings; stable intensity determination instructions for calculating stable intensity values corresponding to the sets of photosite intensity values at the plurality of photosites for the plurality of varying brightness readings; and photon transfer curve approximation instructions for approximating a photon transfer curve corresponding to the photometric sensor based on the statistical dispersions and the stable intensity values, wherein the instrument is adapted to imaging for biological analysis. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
-
Specification