Analyzing instrument, analyzing device, and method of manufacturing analyzing instrument

• US 7,047,795 B2
• Filed: 07/30/2002
• Issued: 05/23/2006
• Est. Priority Date: 08/01/2001
• Status: Expired due to Fees

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Sato, Yoshiharu
• Primary Examiner(s): LARKIN, DANIEL SEAN
• Application Number:
  US10/485,169
• Publication Number:
  US 20040216516A1
• Time in Patent Office: 1,393 Days
• Field of Search: 73/645.6, 422/82.02, 422/98, 204/400, 204/416, 294/28
• US Class Current: 73/64.56
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 29/49826 Assembling or joining

Abstract

An analyzing instrument (2) according to the present invention includes a capillary (6), a sample liquid inlet (42), and a liquid introduction controller for a controlled pattern of introduction of the sample liquid into the capillary. The capillary (6) preferably includes a common passage (60) and a plurality of individual passages (61–63) connecting to the common passage. In this arrangement, the liquid introduction controller selects for each of the individual passages (61–63) whether or not the sample liquid is introduced therein. The liquid introduction controller includes, for example, one or more through-holes (51–53) each communicating with e.g. the individual passages (61–63). The liquid introduction controller preferably selects for each of the individual passages (61–63) whether or not the sample liquid is introduced therein, by a selection whether or not the corresponding one of the through-holes (61–63) is opened or closed.

Claims

1. An analyzing instrument comprising:

a capillary for moving and retaining a sample liquid;

a sample liquid inlet for introduction of the sample liquid into the capillary;

measuring electrodes for measuring a target component in the sample liquid;

liquid introduction regulator for controlled introduction of the sample liquid into the capillary; and

one or more detecting electrodes provided separately from the measuring electrodes for detecting the controlled introduction of the sample liquid into the capillary.

2. The analyzing instrument according to claim 1, further comprising:

a common passage in the capillary; and

a plurality of individual passages in the capillary connecting to the common passage,

wherein the liquid introduction regulator determines for each of the individual passages whether or not the sample liquid is introduced therein.

3. The analyzing instrument according to claim 2, wherein the liquid introduction regulator includes one or more through-holes.

4. The analyzing instrument according to claim 2, wherein the liquid introduction regulator includes a plurality of through-holes,

each through-hole communicating with a corresponding one of the individual passages.

5. The analyzing instrument according to claim 4, wherein the liquid introduction regulator determines for each of the individual passages whether or not the sample liquid is introduced therein, by determining whether or not the corresponding through-hole is opened or closed.

6. The analyzing instrument according to claim 5, wherein each of the plurality of detecting electrodes corresponding to one of the individual passages for detection of the introduction of the sample liquid into the individual passage,

wherein each through-hole is right above the corresponding detecting electrode.

7. The analyzing instrument according to claim 4, wherein the liquid introduction regulator determines for each of the individual passages whether or not the sample liquid is introduced therein, by determining a location at which the corresponding through-hole is formed.

8. The analyzing instrument according to claim 7, wherein each of said one or more detecting electrodes corresponds to one of the individual passages for detection of the introduction of the sample liquid into the individual passage,

wherein each of those through-holes corresponding to the individual passage to which the sample liquid is to be introduced is right above the corresponding detecting electrode,

each of those through-holes corresponding to the individual passage to which the sample liquid is not to be introduced being closer to the sample liquid inlet than is an end on the sample liquid inlet side of the corresponding detecting electrode.

9. The analyzing instrument according to claim 4, wherein the through-holes are formed in a cover laminated to a substrate via a spacer.

10. The analyzing instrument according to claim 2, wherein the capillary is formed by a substrate, a spacer and a cover laminated to the substrate via the spacer,

the spacer including a recess for formation of an inner space of the capillary, and one or more projections extending toward the sample liquid inlet for formation of the individual passages.

11. The analyzing instrument according to claim 1, wherein the sample liquid inlet includes a through-hole, the introduction of the sample liquid being regulated by determining whether or not the through-hole is opened or closed.

12. The analyzing instrument according to claim 1, wherein

the liquid introduction regulator includes one or more through-holes communicating with the capillary, locations in the capillary to which the sample liquid is to be introduced being determined by locations at which said one or more through-holes are formed.

13. The analyzing instrument according to claim 1,

wherein the liquid introduction regulator includes one or more through-holes communicating with the capillary, locations in the capillary to which the sample liquid is to be introduced being determined by whether each of said one or more through-holes is opened or closed.

14. An analyzing device to which an analyzing instrument is attached for analysis of a sample liquid supplied to the analyzing instrument,

the analyzing instrument including:

a capillary for moving and retaining a sample liquid;

a sample liquid inlet for introduction of the sample liquid into the capillary;

measuring electrodes for measuring a specific component of the sample liquid;

a liquid introduction regulator for controlled introduction of the sample liquid into the capillary;

one or more detecting electrodes provided separately from the measuring electrodes for detecting the controlled introduction of the sample liquid into the capillary,

the analyzing device including a detector connected to said one or more detecting electrodes for detection of the controlled introduction of the sample liquid in the capillary.

15. The analyzing device according to claim 14, wherein said one or more detecting electrodes measure an electrical physical quantity for detection purpose as necessary information for the detection of the controlled introduction of the sample liquid in the capillary;

and the measuring electrodes measure an electrical physical quantity for analysis purpose as necessary information for calculating a concentration of the target component in the sample liquid,

to analyzing device further comprising;

a measurer for the measurement of the electrical physical quantity for detection purpose and the electrical physical quantity for analysis purpose;

a storage for storing information about a plurality of calibration curves indicating a relationship between the electrical physical quantity for detection purpose and the concentration of the target component in the sample liquid;

a selector for selection of a target calibration curve, from the plurality of calibration curves; and

an arithmetic processor for calculation of the concentration of the target component based on the electrical physical quantity for analysis purpose and the calibration curve selected by the selector.

16. The analyzing device according to claim 15,

wherein the analyzing instrument further includes:

a common passage in the capillary; and

a plurality of individual passages in the capillary connecting to the common passage, and

wherein the detector detects for each of the individual passages whetter or not the sample liquid has been introduced therein.

17. The analyzing device according to claim 16, wherein each of said one or more detecting electrodes corresponds to one of the individual passages for measurement by the detector of the electrical physical quantity for detection purpose as information necessary for detecting whether or not the sample liquid has been introduced into the individual passage.

18. The analyzing device according to claim 17, wherein the detector makes a determination for each of the electrical physical quantities obtained by the measurer through said one or more detecting electrodes whether or not the quantity has exceeded a threshold value, thereby making a determination whether or not the sample liquid has been introduced for each of the individual passages.

19. The analyzing device according to claim 15, further comprising a plurality of switches for selectively connecting each of said one or more detecting electrodes with the measurer.

20. A method of manufacturing an analyzing instrument comprising:

a capillary for moving and retaining a sample liquid; and

a plurality of through-holes for controlled introduction of the sample liquid into the capillary,

the method including a step of selectively closing the through-holes for providing the controlled introduction of the sample liquid into the capillary.

21. The method of manufacturing the analyzing instrument according to claim 20,

wherein the analyzing instrument further includes:

a sample liquid inlet for introduction of the sample liquid into the capillary, a common passage in the capillary; and

a plurality of individual passages in the capillary connecting to the common passage, each of the individual passages communicating with a corresponding one of the through-holes in the individual passages, and

wherein the step of selectively closing the through-holes includes closing of those through-holes which correspond to those individual passages into which the sample liquid is not to be introduced.

22. A method of manufacturing an analyzing instrument comprising:

a capillary for moving and retaining a sample liquid;

a sample liquid inlet for introduction of the sample liquid into the capillary;

a common passage in the capillary;

a plurality of individual passages located farther away from the sample liquid inlet than is the common passage and connected to the common passage; and

a plurality of through-holes for determining for each of the individual passages whether to introduce or not to introduce the sample liquid there in;

the method including;

a step of forming a first through-hole corresponding to one of the individual passages into which the sample liquid is not to be introduced, closer to an incoming end of said one individual passage for communication with said one individual passage; and

a step of forming a second through-hole corresponding to another of the individual passages into which the sample liquid is to be introduced, farther from an incoming end of said individual passage for communicating with said another individual passage.

23. The method of manufacturing the analyzing instrument according to claim 22, wherein

the analyzing instrument further comprising a plurality of detecting electrodes each corresponding to one of the individual passages for detection of the introduction of the sample liquid into the individual passage,

the first through-hole being formed closer to the incoming end of the individual passage than is an end of the corresponding detecting electrode,

the second through-hole being formed right above the corresponding detecting electrode.