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Method of preparing normalized and/or subtracted cDNA

  • US 7,049,065 B2
  • Filed: 08/24/2001
  • Issued: 05/23/2006
  • Est. Priority Date: 08/25/2000
  • Status: Expired due to Fees
First Claim
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1. A method of preparing normalized and/or subtracted cDNAs comprising the steps of:

  • I) preparing uncloned full-length or full-coding length cDNA tester;

    II) preparing polynucleotide drivers for normalization and/or subtraction;

    III) conducting normalization and/or subtraction and removing tester/driver hybrids, non-specifically hybridized polynucleotide drivers and non-hybridized polynucleotide drivers; and

    IV) recovering the normalized and/or subtracted full-length or full-coding length cDNA.

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