Method of tissue modulation for noninvasive measurement of an analyte
First Claim
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1. A method of noninvasive spectroscopic measurement of an analyte in a subject comprising:
- (a) irradiating tissue of the subject through a nail with electromagnetic radiation having an excitation wavelength while the tissue is in a blood-replete state;
(b) collecting the Raman spectra emitted by the tissue in the blood-replete state;
(c) applying a tissue modulation device having an orifice to the nail of the subject so as to achieve a blood-depleted state within the tissue;
(d) irradiating the tissue through the nail with electromagnetic radiation having an excitation wavelength while the tissue is in the blood-depleted state;
(e) collecting the Raman spectra emitted by the tissue in the blood-depleted state; and
(f) analyzing the collected Raman spectra to determine a concentration of analyte present in the tissue, wherein the analyzing comprises determining the difference between the Raman spectra collected in the blood-replete and blood-depleted states.
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Abstract
Disclosed are a device and methods for obtaining signals related to blood analytes and bone features by collecting spectra, such as fluorescence and Raman spectra, through the nail of a finger or toe. The invention additionally provides methods of modulating blood flow in a tissue of a subject.
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Citations
17 Claims
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1. A method of noninvasive spectroscopic measurement of an analyte in a subject comprising:
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(a) irradiating tissue of the subject through a nail with electromagnetic radiation having an excitation wavelength while the tissue is in a blood-replete state; (b) collecting the Raman spectra emitted by the tissue in the blood-replete state; (c) applying a tissue modulation device having an orifice to the nail of the subject so as to achieve a blood-depleted state within the tissue; (d) irradiating the tissue through the nail with electromagnetic radiation having an excitation wavelength while the tissue is in the blood-depleted state; (e) collecting the Raman spectra emitted by the tissue in the blood-depleted state; and (f) analyzing the collected Raman spectra to determine a concentration of analyte present in the tissue, wherein the analyzing comprises determining the difference between the Raman spectra collected in the blood-replete and blood-depleted states. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of noninvasive spectroscopic measurement of an analyte in a subject comprising:
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(a) irradiating tissue of the subject through a nail with electromagnetic radiation having an excitation wavelength by directing the electromagnetic radiation at the nail side of the finger or toe of the subject; (b) collecting the spectra emitted by the tissue through the nail side of the finger or toe of the subject in blood-replete and blood-depleted states; (c) analyzing collected Raman spectra to determine a signal that is proportional to the amount of analyte present in the tissue by determining the difference between Raman spectra collected in the blood-replete state and the blood-depleted state; (d) analyzing spectra collected in step (b) that are indicative of blood volume; and (e) comparing the spectra analyzed in step (c) with the spectra analyzed in step (d) to determine the amount of analyte per unit blood volume. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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Specification