Thermal cycler with protection from atmospheric moisture

• US 7,051,536 B1
• Filed: 11/12/2004
• Issued: 05/30/2006
• Est. Priority Date: 11/12/2004
• Status: Active Grant

• Current Assignee: Bio-Rad Laboratories Incorporated
• Original Assignee: Bio-Rad Laboratories Incorporated
• Inventors: Banerji, Sunand, Cohen, David A., Denninger, Michael J.
• Primary Examiner(s): Jones, Melvin
• Application Number:
  US10/987,931
• Publication Number:
  US 20060101830A1
• Time in Patent Office: 564 Days
• Field of Search: 62/32, 62/33, 62/37, 622/591, 62/36
• US Class Current: 62/3.3
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1894 Cooling means; Cryo cooling
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: F25B 21/04 reversible
• CPC Class Codes: F25B 2321/023 Mounting details thereof

Abstract

Localized temperature control in a thermal cycler is achieved by thermoelectric modules that are protected from exposure to atmospheric moisture by a pair of loop-shaped gaskets that seal off an enclosure formed by the sample block, the heat sink, and a support frame to which the components are secured. The heat sink is a block with a plurality of fins and is secured to the thermoelectric modules by one or more clamping bars that fit between the fins and are arranged to eliminate interference with the fin geometry and with the functional surface area of the fins. Electric leads are embedded in a molded retainer element, each lead being in the shape of a “U” with two exposed legs joined by a bar at one end, one of the leads extending into the region sealed from the atmosphere and the other extending outside the region.

Claims

1. Apparatus for controlling temperature in a plurality of samples, said apparatus comprising:

a multi-receptacle sample block, a thermoelectric module, and a heat sink block, all shaped to be capable of arrangement in a stacked configuration in which said sample block is in thermal contact with said thermoelectric module and said thermoelectric module is in thermal contact with said heat sink block,

a support frame sized to receive said sample block, said thermoelectric module, and said heat sink block in said stacked configuration,

a first loop-shaped gasket sized to encircle said sample block along a peripheral surface thereof and thereby form a seal between said sample block and said support frame, and

a second loop-shaped gasket sized to encircle said heat sink block along a peripheral surface thereof and thereby form a seal between said heat sink block and said support frame,

said sample block, said heat sink block, said support frame, and said loop-shaped gaskets thereby together forming a sealed enclosure surrounding said thermoelectric module and protecting said thermoelectric module from atmospheric moisture.

2. The apparatus of claim 1 further comprising a first groove in said peripheral surface of said sample block to receive said first loop-shaped gasket and a second groove in said peripheral surface of said heat sink block to receive said second loop-shaped gasket, and wherein said support frame has flat surfaces where said support frame contacts said first and second loop-shaped gaskets.

3. The apparatus of claim 1 comprising a plurality of thermoelectric modules arranged edge to edge in a planar array, and wherein in said stacked configuration said sample block is in thermal contact with said planar array, and said planar array is in thermal contact with said heat sink block.

4. The apparatus of claim 1 wherein said sealed enclosure defines a gap between edges of said thermoelectric module and said support frame.

5. The apparatus of claim 1 wherein said support frame is a unitary molded piece with electric leads partially embedded therein.

6. The apparatus of claim 5 wherein said sealed enclosure defines a gap between edges of said thermoelectric module and said support frame and said electric leads have exposed ends extending into said gap.

7. The apparatus of claim 5 wherein each electric lead consists of first and second legs joined by a cross-bar in a U-shape, said cross-bar embedded in said molded piece and said first and second legs exposed, said first leg extending inside said sealed enclosure and said second leg extending outside said sealed enclosure.

8. A molded housing for an electronic instrument, said housing comprising a sealed enclosure comprising:

a molded partition separating an interior from atmospheric exposure, and

a U-shaped electric lead defined by first and second legs joined by an end cross-bar, said end cross-bar embedded in said partition with said first leg extending to one side of said partition and said second leg extending to another side of said partition.

9. Apparatus for controlling temperature in a plurality of samples, said apparatus comprising:

a multi-receptacle sample block, a thermoelectric module, and a finned heat sink block, all shaped to be capable of arrangement in a stacked configuration in which said sample block is in thermal contact with said thermoelectric module and said thermoelectric module is in thermal contact with said heat sink block,

a support frame sized to receive said sample block, said thermoelectric module, and said finned heat sink block in said stacked configuration, and

a bar greater in length than said fins of said finned heat sink block and sized to fit between adjacent fins of said finned heat sink block, and spring-loaded fasteners affixing said bar to said support frame to secure said finned heat sink block against said thermoelectric module when in said stacked configuration, one such fastener at each end of said bar.

10. Apparatus for controlling temperature in a plurality of samples, said apparatus comprising:

a multi-receptacle sample block, a thermoelectric module, and a finned heat sink block, all shaped to be capable of arrangement in a stacked configuration in which said sample block is in thermal contact with said thermoelectric module and said thermoelectric module is in thermal contact with said heat sink block,

a support frame sized to receive said sample block, said thermoelectric module, and said finned heat sink block in said stacked configuration, and

exactly two bars sized to fit between adjacent fins of said finned heat sink block, and a pair of spring-loaded fasteners positioned on opposing ends of said fins, each said fastener engaging both bars.