Method for highly sensitive hybridization of nucleic acids, and method for gene analysis using the same
First Claim
1. A method for hybridizing nucleic acids, which comprises an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with a fluorophore or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step, characterized in that said complementary double strand is formed by a temperature gradient processing from a high temperature area to a low temperature area in said annealing step, and an endonuclease is added to recognize and remove a non- complementary nucleic acid portion contained in said complementary double strand in said enzyme treatment step.
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Abstract
A method for hybridizing nucleic acids, which includes an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with fluorescence or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step. In the annealing step, the complementary double strand is formed by performing a temperature gradient processing performed from a high temperature area to a low temperature area, and in the enzyme treatment step, a noncomplementary nucleic acid portion contained in the complementary double stand is recognized and cleaved by adding endonuclease. This method has high measurement sensitivity and the operation is simple.
7 Citations
6 Claims
- 1. A method for hybridizing nucleic acids, which comprises an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with a fluorophore or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step, characterized in that said complementary double strand is formed by a temperature gradient processing from a high temperature area to a low temperature area in said annealing step, and an endonuclease is added to recognize and remove a non- complementary nucleic acid portion contained in said complementary double strand in said enzyme treatment step.
- 2. A method for hybridizing nucleic acids, which comprises an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with a fluorophore or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step, characterized in that said complementary double strand is formed by a pH gradient processing from a high pH area to a low pH area in said annealing step, and an endonuclease is added to recognize and remove a non-complementary nucleic acid portion contained in said complementary double strand in said enzyme treatment step.
- 3. A method for hybridizing nucleic acids, which comprises an annealing step of preparing a first single stranded nucleic acid fragment immobilized on a surface of an immobilizing material and a second single stranded nucleic acid fragment labeled with a fluorophore or radioisotope and forming a complementary double strand from the first single stranded nucleic acid fragment and the second single stranded nucleic acid fragment, and an enzyme treatment step, characterized in that said complementary double strand is formed by a salt concentration gradient processing from a low salt concentration area to a high salt concentration area in said annealing step, and an endonuclease is added to recognize and remove a non-complementary nucleic acid portion contained in said complementary double strand in said enzyme treatment step.
Specification
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- Resources
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Current AssigneeNGK Insulators Limited
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Original AssigneeNGK Insulators Limited
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InventorsYamada, Kazunari, Kawase, Mitsuo, Miyazaki, Isao, Matsuda, Chiho
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Primary Examiner(s)Whisenant, Ethan
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Application NumberUS10/258,344Publication NumberTime in Patent Office1,551 DaysField of Search435/6, 536/23.1, 536/24.3US Class Current435/6.16CPC Class CodesC12Q 1/6827 for detection of mutation o...C12Q 2521/301 EndonucleaseC12Q 2527/101 Temperature
