Bioanalytical measuring method using oxidases and lanthanoid-ligand complexes
First Claim
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1. Method for determining enzymatically generated hydrogen peroxide, comprising determining with the aid of a lanthanoid-ligand complex, hydrogen peroxide that is formed by an enzyme that is a hydrogen peroxide-generating oxidase, wherein the hydrogen peroxide formed by oxidase activity results in a measurable change in light absorption in a wavelength range between 200 nm and 500 nm or in fluorescent properties of the lanthanoid-ligand complex.
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Abstract
The present invention concerns biological methods of determination using enzymes from the group of oxidases and using optical indicators from the group of lanthanoid-ligand complexes.
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26 Claims
- 1. Method for determining enzymatically generated hydrogen peroxide, comprising determining with the aid of a lanthanoid-ligand complex, hydrogen peroxide that is formed by an enzyme that is a hydrogen peroxide-generating oxidase, wherein the hydrogen peroxide formed by oxidase activity results in a measurable change in light absorption in a wavelength range between 200 nm and 500 nm or in fluorescent properties of the lanthanoid-ligand complex.
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