×

Method for sequencing nucleic acid molecules

  • US 7,056,661 B2
  • Filed: 05/17/2000
  • Issued: 06/06/2006
  • Est. Priority Date: 05/19/1999
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of obtaining nucleotide sequence information for a target nucleic acid molecule having a plurality of nucleotide bases, said method comprising:

  • providing a single complex of a nucleic acid polymerizing enzyme and the target nucleic acid molecule oriented with respect to each other in a position suitable to add a nucleotide analog at an active site complementary to the target nucleic acid, wherein the complex is immobilized to a solid support;

    providing a plurality of types of nucleotide analogs that lack a blocking substituent proximate to the active site, each type of nucleotide analog being complementary to a different nucleotide in the target nucleic acid and having a different detectable moiety, and polymerizing a nucleotide analog at the active site, wherein the added nucleotide analog is complementary to the nucleotide of the target nucleic acid and is capable of polymerization with a subsequent nucleotide analog;

    identifying the nucleotide analog added at the active site as a result of said polymerizing by detecting its detectable label, wherein said identifying is carried out by directing activating radiation to a region corresponding to the active site and detecting the detectable label from the nucleotide analog added to the active site by a technique which reduces background noise resulting from free unpolymerized nucleotide analogs; and

    repeating, with the complex immobilized to the solid support, said providing a plurality of types of nucleotide analogs, said polymerizing, and said identifying, so that nucleotide analogs added at downstream active sites are identified and, as a result, nucleotide sequence information for the target nucleic acid is determined.

View all claims
  • 3 Assignments
Timeline View
Assignment View
    ×
    ×