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Analysis of surface immobilized polymers utilizing microfluorescence detection

DC
  • US 7,056,666 B2
  • Filed: 02/14/2002
  • Issued: 06/06/2006
  • Est. Priority Date: 12/06/1990
  • Status: Expired due to Fees
First Claim
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1. A method for determining the positions of polynucleotides which terminate with a given monomer, comprisingproviding a plurality of different polynucleotides attached at distinct positions of a surface of a support by annealing of the polynucleotides to oligonucleotides immobilized to regions of the surface of the support at a density of at least 1000 regions/cm2, the polynucleotides having terminal monomers labeled in a monomer-type specific manner;

  • andscanning said surface, thereby determining the positions of said labeled monomer.

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