Preservation of RNA in a biological sample
First Claim
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1. A method of analyzing a biological sample comprising:
- precipitating RNA in the biological sample by incubating the biological sample with an RNA preservative in an aqueous solution to yield a biological sample containing preserved RNA;
histochemically staining the biological sample containing preserved RNA;
histochemically analyzing the stained biological sample containing preserved RNA to identify specific cell populations; and
analyzing mRNA expression patterns of the identified cells by a method comprising;
in-situ hybridization;
or isolating identified cells and subjecting the isolated cells to bioarray gene profiling.
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Abstract
To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods.
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4 Claims
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1. A method of analyzing a biological sample comprising:
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precipitating RNA in the biological sample by incubating the biological sample with an RNA preservative in an aqueous solution to yield a biological sample containing preserved RNA; histochemically staining the biological sample containing preserved RNA; histochemically analyzing the stained biological sample containing preserved RNA to identify specific cell populations; and analyzing mRNA expression patterns of the identified cells by a method comprising;
in-situ hybridization;
or isolating identified cells and subjecting the isolated cells to bioarray gene profiling. - View Dependent Claims (2, 3, 4)
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Specification