Urate oxidase

• US 7,056,713 B1
• Filed: 08/05/1999
• Issued: 06/06/2006
• Est. Priority Date: 08/06/1998
• Status: Expired due to Term

• Current Assignee: Duke University
• Original Assignee: Duke University
• Inventors: Kelly, Susan J., Hershfield, Michael
• Primary Examiner(s): Patterson, Jr., Charles L.
• Application Number:
  US09/762,097
• Time in Patent Office: 2,497 Days
• Field of Search: 435/189, 435/320.1, 435/252.3, 536/23.2
• US Class Current: 435/189
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 19/06 Antigout agents, e.g. antihyperuricemic or uricosuric agents
• CPC Class Codes: C12N 9/0046 with oxygen as acceptor (1.7.3)
• CPC Class Codes: C12N 9/0048 Uricase (1.7.3.3)
• CPC Class Codes: C12Y 107/03003 Factor-independent urate hydroxylase (1.7.3.3), i.e. uricase

Abstract

The present invention relates, in general, to urate oxidase (uricase) proteins and nucleic acid molecules encoding same. In particular, the invention relates to uricase proteins which are particularly useful as, for example, intermediates for making improved modified uricase proteins with reduced immunogenicity and increased bioavailability.

Claims

1. A protein comprising a recombinant uricase chimeric protein which comprises 304 amino acids, the first 225 N-terminal portion of said 304 amino acids being amino acids 1–

225 of porcine uricase and the remaining 79 amino acids of said 304 amino acids being amino acids 226–

304 of baboon uricase.

4. An isolated and purified nucleic acid molecule coding for the recombinant uricase of claim 1.

2. A protein comprising a recombinant uricase chimeric protein which comprises 304 amino acids, the first 288 N-terminal portion of said 304 amino acids being amino acids 1–

288 of porcine uricase and the remaining 16 amino acids of said 304 amino acids being amino acids 289–

304 of baboon uricase.

5. An isolated and purified nucleic acid molecule coding for the recombinant uricase of claim 2.

3. A recombinant uricase protein selected from the group consisting of SEQ ID NO:

s 2, 4, 8, 9, 10 and 11.

6. An isolated and purified nucleic acid molecule coding for the recombinant uricase of claim 3.

7. An isolated and purified nucleic acid molecule of claim 6 having a base sequence of SEQ ID NO:

1.

8. An isolated and purified nucleic acid molecule of claim 6 having a base sequence of SEQ ID NO:

3.

9. A vector comprising a nucleic acid molecule of claim 6.

10. A host cell comprising a vector according to claim 9.