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Nucleotide compositions comprising photocleavable markers and methods of preparation thereof

  • US 7,057,031 B2
  • Filed: 07/12/2002
  • Issued: 06/06/2006
  • Est. Priority Date: 07/13/2001
  • Status: Expired due to Fees
First Claim
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1. A method of preparing a marker-photocleavable linker-nucleotide conjugate comprising:

  • a) providing i) a photocleavable linker comprising a protective group, ii) an amino-nucleotide analog, and iii) an activated marker molecule selected from the group consisting of BODIPY-FL-SF and Cy5-NHS;

    b) operably linking said photocleavable linker to said amino-nucleotide analog to produce a photocleavable linker-nucleotide conjugate;

    c) removing said protective group from said photocleavable linker-nucleotide conjugate under conditions such that an activated photocleavable linker-nucleotide conjugate is created, wherein said activated photocleavable linker-nucleotide conjugate has an exposed reactive site on the linker portion of the conjugate; and

    d) contacting said activated marker molecule with said activated photocleavable linker-nucleotide conjugate under conditions such that a marker-photocleavable linker-nucleotide conjugate is produced.

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