System and method for programmable illumination pattern generation

• US 7,057,704 B2
• Filed: 03/16/2002
• Issued: 06/06/2006
• Est. Priority Date: 09/17/2000
• Status: Expired due to Term

• Current Assignee: BioArray Solutions Limited (Werfen SA)
• Original Assignee: BioArray Solutions Limited (Werfen SA)
• Inventors: Seul, Michael, Chau, Chiu Wo
• Primary Examiner(s): Kim, Peter B.
• Application Number:
  US10/098,604
• Publication Number:
  US 20020198665A1
• Time in Patent Office: 1,543 Days
• Field of Search: 355/53, 355/35, 355/67, 435/6, 435/7.2, 436/518, 359/855, 427/586, 205/666, 430/320, 382/312, 349/5
• US Class Current: 355/53
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/089 Virtual walls for guiding liquids
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 2021/6478 Special lenses
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

An apparatus providing programmable illumination pattern generation for the manipulation of colloidal particulates and biomolecules in suspension between electrodes, is disclosed. The apparatus implements LEAPS (Light-controlled electrokinetic assembly of particles near surfaces), which relies on: AC electric field-induced assembly of particles: the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The apparatus generates patterns of illumination and projects them on to planar surfaces. i.e., a LEAPS electrode. This enables the creation of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (“time-varying patterns”), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest, to provide for arrangements and assembly of particles in such patterns.

Claims

1. An apparatus for programmably generating an illumination pattern superimposed on an electrode, said electrode displaying lowered impedance in regions or its surface where it is illuminated, said illumination pattern having a predetermined arrangement of light and dark zones, said apparatus comprising:

a first substantially planar electrode in substantially parallel arrangement with another substantially planar electrode, with said electrodes being serrated by a gap, and the gap containing an electrolyte solution which is in contact with said electrodes and which contains suspended colloidal particles;

an illumination source capable of selectively illuminating a surface of the electrode, whereby upon application of an AC voltage to the electrode, the electric field in the illuminated regions of the surface is greater than in the non-illuminated regions of the surface, and wherein the illumination pattern is projected onto said first electrode so as to control the assembly and lateral motion of said colloidal particles, said assembly and lateral motion being induced by the AC voltage applied between said electrodes;

a reconfigurable mask composed of an array of pixels, said pixels being actively controllable and directly addressable by a computer-controlled circuit and computer interface, said computer-controlled circuit providing temporal control of the intensity of illumination emanating from said pixels so as to form the illumination pattern;

a projection system suitable for imaging the reconfigurable mask onto the electrode; and

an imaging and recording system capable of viewing and recording said electrode with superimposed illumination pattern.

2. The apparatus of claim 1, further comprising an image analysis system permitting acquisition of digitized images of the illumination pattern, analysis of said digitized images so as to extract features of interest, and thereby to permit creation of derivative patterns based on said features of interest.

3. The apparatus of claim 1, wherein said computer-controlled circuit and computer interface are capable of accepting input from a video display adapter.

4. The apparatus of claim 1, wherein said array of pixels is actively controlled so as to permit adjustment of variable and controllable levels of pixel transmissivity or reflectivity.

5. The apparatus of claim 4, wherein said array of pixels comprises a liquid crystal display or a digital micromirror device.

6. The apparatus of claim 1, wherein said software program provides a series of illumination patterns, said patterns being produced interactively in a graphical user interface software program or being replayed from a storage device containing previously produced patterns.

7. An apparatus for programmably reconfiguring an array of particles on a light-sensitive planar electrode by programmable adjustment of an illumination pattern projected onto the light-sensitive planar electrode comprising:

an illumination source;

a reconfigurable mask composed of an array of pixels, said pixels being actively controllable and directly addressable by means of a computer-controlled circuit and computer interface, said computer-controlled circuit being operated using a software program providing temporal control of the intensity of illumination emanating from each pixel so as to form the illumination pattern comprising the predetermined arrangement of light and dark zones;

a projection system suitable for imaging the reconfigurable mask onto the light-sensitive planar electrode, wherein the light-sensitive planar electrode, upon illumination, displays lowered impedance in the illuminated regions, said light-sensitive planar electrode being aligned with another planar electrode in substantially parallel arrangement, with said electrodes being separated by a gap and the gap containing an electrolyte solution which is in contact with said electrodes and which contains colloidal particles suspended in the electrolyte solution; and

an imaging system incorporating a camera capable of viewing said light-sensitive planar electrode.

8. A programmable patterning device for generating a chemically patterned surface or surface coating of an electrode comprising:

an apparatus for programmably generating an illumination pattern having a predetermined arrangement of light and dark zones on said surface, the apparatus comprising;

an illumination source;

a reconfigurable mask composed of an array of pixels, said pixels being actively controllable and directly addressable by means of a computer-controlled circuit and computer interface, said computer-controlled circuit being operated using a software program providing temporal control of the intensity of illumination emanating from each pixel so as to form the illumination pattern comprising the predetermined arrangement of light and dark zones;

a projection system suitable for imaging the reconfigured mask onto the surface;

an imaging system incorporating a camera capable of viewing said electrode with superimposed illumination pattern; and

upon illumination by light of pre-selected spectral composition of the electrode surface or surface coating, a physical or chemical property of the surface or surface coating is altered in accordance with the illumination pattern.

9. The device of claim 8, wherein the physical or chemical property is solubility in a pre-selected solvent, and whereby the chemically patterned surface or surface coating is generated following illumination and upon exposure of the surface to said solvent.

10. The device of claim 8, wherein the physical or chemical property is chemical reactivity and wherein there is subsequent functionalization, following illumination, of the surface by chemical reaction.

11. The device of claim 8, wherein the spectral composition contains the wavelength of the visible spectrum.