Methods and systems for reducing background signal in assays
First Claim
Patent Images
1. A method of monitoring a reaction, comprising:
- providing a first reaction mixture in a first fluidic conduit, the first reaction mixture comprising;
a first reagent having a detectable label associated therewith;
a second reagent that reacts with the first reagent to produce a first product having the detectable label associated therewith, the first product having an electrophoretic mobility that is different from the first reagent;
providing a detection zone in a second fluidic conduit which is fluidly coupled to the first fluidic conduit at a first intersection,directing the first reaction mixture along a length of the first fluidic conduit;
controllably directing the first product and substantially no first reagent into the second fluidic conduit away from the first intersection; and
detecting the product at the detection zone in the second fluidic conduit.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods and systems of monitoring reactions, e.g., assays, that filter out background signal from substrate, in detecting the product. The methods and systems controllably move detectable reaction product from a first location to a second location at which the product is detected while controllably moving potentially interfering substrate materials away from or not toward the second location. Controllable movement of the different species is accomplished through the controlled combination of bulk fluid flow and differential electrophoresis of substrate and product to move the product, but not the substrate past the detection region.
153 Citations
13 Claims
-
1. A method of monitoring a reaction, comprising:
-
providing a first reaction mixture in a first fluidic conduit, the first reaction mixture comprising; a first reagent having a detectable label associated therewith; a second reagent that reacts with the first reagent to produce a first product having the detectable label associated therewith, the first product having an electrophoretic mobility that is different from the first reagent; providing a detection zone in a second fluidic conduit which is fluidly coupled to the first fluidic conduit at a first intersection, directing the first reaction mixture along a length of the first fluidic conduit; controllably directing the first product and substantially no first reagent into the second fluidic conduit away from the first intersection; and detecting the product at the detection zone in the second fluidic conduit. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
Specification