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Methods and systems for reducing background signal in assays

  • US 7,060,171 B1
  • Filed: 07/24/2002
  • Issued: 06/13/2006
  • Est. Priority Date: 07/31/2001
  • Status: Active Grant
First Claim
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1. A method of monitoring a reaction, comprising:

  • providing a first reaction mixture in a first fluidic conduit, the first reaction mixture comprising;

    a first reagent having a detectable label associated therewith;

    a second reagent that reacts with the first reagent to produce a first product having the detectable label associated therewith, the first product having an electrophoretic mobility that is different from the first reagent;

    providing a detection zone in a second fluidic conduit which is fluidly coupled to the first fluidic conduit at a first intersection,directing the first reaction mixture along a length of the first fluidic conduit;

    controllably directing the first product and substantially no first reagent into the second fluidic conduit away from the first intersection; and

    detecting the product at the detection zone in the second fluidic conduit.

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