Method for fragmenting and labeling DNA involving abasic sites and phosphate labeling
First Claim
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1. A method for fragmenting and labeling a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:
- fragmenting the DNA by creating at least one abasic site on said DNA,attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment.
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Abstract
A single- or double-stranded deoxyribonucleic acid (DNA) is fragmented and labeled by chemically fragmenting the DNA by creating at least one abasic site on the DNA, and attaching a marker to at least one of the fragments by means of a labeling reagent, the reagent covalently and predominantly coupling to at least one phosphate of the fragment. This method can be applied in the field of diagnosis.
36 Citations
31 Claims
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1. A method for fragmenting and labeling a single- or double-stranded deoxyribonucleic acid (DNA) comprising the following steps:
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fragmenting the DNA by creating at least one abasic site on said DNA, attaching a marker to at least one of the fragments by means of a labeling reagent, said reagent covalently and predominantly coupling to at least one phosphate of said fragment. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification