Evaluating binding affinities by force stratification and force panning
First Claim
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1. A method for determining a binding affinity between an object and one or more materials using a scanning probe microscope comprising:
- (a) obtaining a surface, the surface further comprising one or more materials deposited thereon;
(b) affixing at least one object to the one or more materials deposited on the surface;
(c) applying a force with a tip of the scanning probe microscope to a first object to remove the first object from a first material, wherein the first object is displaced from the first material and the tip; and
(d) calculating the binding affinity between the first object and the first material from the force applied to remove the object from the first material.
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Abstract
The present invention is a method for selectively removing objects from a surface utilizing a probe. The probe is scanned over the surface utilizing a greater and greater relative amount of force so that a certain number of the objects are removed from the surface. The force required to remove the objects from the surface can be calculated utilizing Hook'"'"'s law and the spring constant of the probe. After removal of the objects that have a relatively weaker binding affinity with the surface, the remaining objects can be harvested, characterized, and subjected to further study.
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14 Claims
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1. A method for determining a binding affinity between an object and one or more materials using a scanning probe microscope comprising:
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(a) obtaining a surface, the surface further comprising one or more materials deposited thereon; (b) affixing at least one object to the one or more materials deposited on the surface; (c) applying a force with a tip of the scanning probe microscope to a first object to remove the first object from a first material, wherein the first object is displaced from the first material and the tip; and (d) calculating the binding affinity between the first object and the first material from the force applied to remove the object from the first material. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method for separating objects based on relative binding affinities using a scanning probe microscope comprising:
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(a) depositing at least one material on a surface; (b) affixing a plurality of objects tote at least one material deposited on the surface; and (c) applying a first force to the surface with a tip of the scanning probe microscope such tat a first object is removed from a first material deposited on the surface, wherein the first object is displaced from the first material and the tip, and wherein a second object is retained on the first material or on a second material deposited on the surface. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14)
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Specification