Method and kit for the determination of analyte concentration in blood

• US 7,060,453 B1
• Filed: 08/19/1999
• Issued: 06/13/2006
• Est. Priority Date: 08/21/1998
• Status: Expired due to Term

• Current Assignee: Falk Fish
• Original Assignee: Falk Fish
• Inventors: Fish, Falk
• Primary Examiner(s): Smith, Lynette R. F.
• Assistant Examiner(s): HINES, JANA A
• Application Number:
  US09/763,415
• Time in Patent Office: 2,490 Days
• Field of Search: 435/2, 435/4, 435/7.1, 435/72, 435/7.24, 435/725, 435/7.8, 435 792- 795, 435/8, 435/12, 435/14, 435/28, 435/183, 435/69.6, 435/283.1, 435/287.1, 435/288.1, 436/14, 436/66, 436/164, 436/172, 436/177, 436/519, 436/520, 436/521, 436/522, 436/807, 436/808, 436/810, 424/278.1
• US Class Current: 435/14
• CPC Class Codes: G01N 33/50 Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/721 Haemoglobin
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A method is provided for determining the level of an analyte in the blood of an individual based on determination of the level of the same analyte in non-blood sample (e.g. urine, saliva and hair) obtained from the individual. The non-blood sample contains red blood cells and the volume of the blood in the sample together with the amount of the analyte in the sample are the basis for calculating the level of the analyte in the individual'"'"'s blood. Kits for carrying out the above method are also provided.

Claims

1. A method for determining the level of glucose in blood from a non-blood sample from an individual comprising:

(i) obtaining a sample of hair or urine from said individual, said sample being a non-blood sample but containing blood components;

(ii) determining the volume of blood in the obtained sample by measuring the level of hemoglobin in said sample;

(iii) determining the amount of glucose in the sample or in blood cells present in said non-blood sample; and

(iv) calculating the level of glucose in the blood of the individual based on the measurements in (iii) and (iv).

2. The method according to claim 1, wherein said non-blood sample is a sample of hair obtained from said individual, the method comprising:

(i) obtaining a sample of hair from said individual;

(ii) determining the amount of blood and interstitial fluid, if present, in said obtained sample and if necessary, correcting variations between different hair samples;

(iii) determining the level of glucose in said blood and interstitial fluid, if present and

(iv) calculating the level of glucose in the blood of the tested individual based on the measurements in (ii) and (iii).

3. The method according to claim 2 wherein before stage (ii) said blood and interstitial fluid, if present, are first extracted from a hair follicle of said obtained hair.