Probe for constructing probe-polymer method of constructing probe-polymer and utilization thereof
First Claim
Patent Images
1. Probes for forming a probe-polymer comprising a first probe and a second probe having the following characteristics (a), (b), (c), and (d):
- (a) the first and second probes each comprising n base sequence regions complementary to each other, wherein an X1 region, an X2 region, an X3 region, . . . an Xn region provided in this order from the 5′
-terminal of the first probe have base sequences complementary respectively to an X1′
region, an X2′
region, an X3′
region, . . . an Xn′
region provided in this order from the 5′
terminal of the second probe, wherein n is at least 3;
(b) when the first and second probes are reacted with each other, the X1 region hybridizes only to the X1′
region, the X2 region hybridizes only to the X2′
region, the X3 region hybridizes only to the X3′
region, . . . and the Xn region hybridizes only to the Xn′
region, and when both the probes are bound, they hybridize to each other at any one of the regions in one probe, and a plurality of the first and second probes bound at the one region hybridize to each other to form a probe-polymer;
(c) at least one G (guanine) or C (cytosine) is arranged at both terminals of each one of the complementary base sequence regions in the first and second probes, and upon hybridization of the first and second probes, at least one C—
G bond is formed at all of the terminals of the complementary regions; and
(d) the number of bases in each complementary base sequence region in the first and second probes is at least 8.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a method for measuring a target gene under isothermal conditions without using enzyme. A pair of probes each having n (n≧3) base sequence regions complementary to each other are hybridized alternately to form a double-stranded probe-polymer. A base pair at branched sites of each complementary base sequence region is designed to be a G (guanine)-C (cytosine) bond, whereby a stable double-stranded probe-polymer is formed. One of complementary portions in one probe is constituted to have a base sequence complementary to a part of a target gene, whereby a target gene-probe-polymer complex is formed and the target gene is measured.
-
Citations
7 Claims
-
1. Probes for forming a probe-polymer comprising a first probe and a second probe having the following characteristics (a), (b), (c), and (d):
-
(a) the first and second probes each comprising n base sequence regions complementary to each other, wherein an X1 region, an X2 region, an X3 region, . . . an Xn region provided in this order from the 5′
-terminal of the first probe have base sequences complementary respectively to an X1′
region, an X2′
region, an X3′
region, . . . an Xn′
region provided in this order from the 5′
terminal of the second probe, wherein n is at least 3;(b) when the first and second probes are reacted with each other, the X1 region hybridizes only to the X1′
region, the X2 region hybridizes only to the X2′
region, the X3 region hybridizes only to the X3′
region, . . . and the Xn region hybridizes only to the Xn′
region, and when both the probes are bound, they hybridize to each other at any one of the regions in one probe, and a plurality of the first and second probes bound at the one region hybridize to each other to form a probe-polymer;(c) at least one G (guanine) or C (cytosine) is arranged at both terminals of each one of the complementary base sequence regions in the first and second probes, and upon hybridization of the first and second probes, at least one C—
G bond is formed at all of the terminals of the complementary regions; and(d) the number of bases in each complementary base sequence region in the first and second probes is at least 8. - View Dependent Claims (2, 3)
-
-
4. A reagent for detecting a target gene in a sample, comprising a first probe and a second probe as polymerization probes having the following characteristics (a), (b), (c), (d), and (e) as essential elements:
-
(a) the first and second probes each comprising n base sequence regions complementary to each other, wherein an X1 region, an X2 region, an X3 region, . . . an Xn region provided in this order from the 5′
-terminal of the first probe have base sequences complementary respectively to an X1′
region, an X2′
region, an X3′
region, . . . an Xn′
region provided in this order from the 5′
terminal of the second probe, wherein n is at least 3;(b) when the first and second probes are reacted with each other, the X1 region hybridizes only to the X1′
region, the X2 region hybridizes only to the X2′
region, the X3 region hybridizes only to the X3′
region, . . . and the Xn region hybridizes only to the Xn′
region, and when both the probes are bound, they hybridize to each other at any one of the regions in one probe, and a plurality of the first and second probes bound at the one region hybridize to each other to form a probe-polymer;(c) at least one G (guanine) or C (cytosine) is arranged at both terminals of each one of the complementary base sequence regions in the first and second probes, and upon hybridization of the first and second probes, at least one C—
G bond is formed at all of the terminals of the complementary regions;(d) one of the complementary base sequence regions in either one of the first or second probe has a region having a base sequence complementary to a part of the target gene; and (e) the number of bases in each complementary base sequence region in the first and second probes is at least 8. - View Dependent Claims (6, 7)
-
-
5. A reagent for detecting a target gene in a sample, comprising:
- a first and a second probe having the following characteristics (a), (b), (c), and (d) as polymerization probes; and
at least one target gene capture probe comprising at least two regions, one region of which is a base sequence region complementary to a part of the target gene and the other region of which is a base sequence region complementary to one region in either one of the two polymerization probes as essential elements,(a) the first and second probes each comprising n base sequence regions complementary to each other, wherein an X1 region, an X2 region, an X3 region, . . . an Xn region provided in this order from the 5′
-terminal of the first probe have base sequences complementary respectively to an X1′
region, an X2′
region, an X3′
region, . . . an Xn′
region provided in this order from the 5′
terminal of the second probe, wherein n is at least 3;(b) when the first and second probes are reacted with each other, the X1 region hybridizes only to the X1′
region, the X2 region hybridizes only to the X2′
region, the X3 region hybridizes only to the X3′
region, . . . and the Xn region hybridizes only to the Xn′
region, and when both the probes are bound, they hybridize to each other at any one of the regions in one probe, and a plurality of the first and second probes bound at the one region hybridize to each other to form a probe-polymer;(c) at least one G (guanine) or C (cytosine) is arranged at both terminals of each one of the complementary base sequence regions in the first and second probes, and upon hybridization of the first and second probes, at least one C—
G bond is formed at all of the terminals of the complementary regions; and(d) the number of bases in each complementary base sequence region in the first and second probes is at least 8.
- a first and a second probe having the following characteristics (a), (b), (c), and (d) as polymerization probes; and
Specification
-
- Resources
Litigation Campaign Assessment
Thank you for your request. You will receive a custom alert email when the Litigation Campaign Assessment is available.
×
-
Current AssigneeEIDIA Co., Ltd. (Sekisui)
-
Original AssigneeSanko Junyaku Co., Ltd.
-
InventorsHakii, Chikako, Mitsuka, Mari, Usui, Mitsugu
-
Primary Examiner(s)SITTON, JEHANNE SOUAYA
-
Application NumberUS09/979,999Publication NumberTime in Patent Office1,842 DaysField of Search435/6, 536/23.1, 536/24.3US Class Current536/24.3CPC Class CodesC12N 15/10 Processes for the isolation...C12Q 1/6811 Selection methods for produ...C12Q 1/6816 characterised by the detect...C12Q 1/682 Signal amplificationC12Q 2525/161 incorporating target specif...C12Q 2525/185 incorporating bases where t...C12Q 2525/313 Branched oligonucleotidesC12Q 2527/101 TemperatureC12Q 2537/143 Multiplexing, i.e. use of m...C12Q 2563/179 the label being a nucleic acid
