Isotopic gas analyzer

• US 7,063,667 B1
• Filed: 09/17/1998
• Issued: 06/20/2006
• Est. Priority Date: 09/17/1997
• Status: Expired due to Term

• Current Assignee: BREATHID (2006)
• Original Assignee: Oridion BreathID Ltd.
• Inventors: Ben-Oren, Ilan, Carlebach, Ephraim, Levitsky, Gershon, Giron, Boaz, Colman, Lewis
• Primary Examiner(s): Nasser, Robert L.
• Application Number:
  US09/508,805
• Time in Patent Office: 2,833 Days
• Field of Search: 600/532, 600 32-324, 73/232, 73/232.1, 73/233, 422/83, 422/84, 42/84
• US Class Current: 600/532
• CPC Class Codes: A61B 2560/0252 using ambient temperature
• CPC Class Codes: A61B 2560/0257 using atmospheric pressure
• CPC Class Codes: A61B 5/0836 Measuring rate of CO2 production
• CPC Class Codes: A61B 5/097 Devices for facilitating collection of breath or for directing breath into or through measuring devices
• CPC Class Codes: G01N 21/3504 for analysing gases, e.g. multi-gas analysis
• CPC Class Codes: G01N 2201/0631 Homogeneising elements
• CPC Class Codes: G01N 33/0006 Calibrating gas analysers
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath

Abstract

An NDIR spectrometer based on the use of wavelength specific lamp sources, whose emission spectrum consists of discrete, narrow lines characteristic of the isotope present in the lamp, and which it is desired to measure with the spectrometer. This allows very high intrinsic sensitivity, enabling the use of an extremely compact absorption cell with a very short path length. In addition, the source can be self-modulated, such that problems associated with external choppers are avoided. Furthermore, there is insignificant cross sensitivity between the isotopes themselves and between the isotopes and other ambient gases in the operating environment.

Claims

1. An isotopic gas analyzer for determining the ratio of at least a first and a second isotopic component of a gas, comprising:

at least one wavelength-stable source of radiation of wavelengths characteristic of at least one of said at least first and second isotopic components;

at least a first sample chamber comprising a first sample of said gas to be analyzed;

at least a first reference chamber containing a reference gas comprising said first and second isotopic components;

at least a second sample chamber comprising a second sample of said gas to be analyzed;

at least a second reference chamber containing a reference gas comprising said first and second isotopic components; and

at least one detector detecting transmission through said at least first sample chamber and transmission through said at least first reference chamber, of radiation of wavelengths characteristic of said first isotopic component of said gas, and transmission through said at least second sample chamber and transmission through said at least second reference chamber, of radiation of wavelengths characteristic of said second isotopic component of said gas.

2. An isotopic gas analyzer according to claim 1, and wherein said at least one detector is a single detector.

3. An isotopic gas analyzer according to claim 2, and wherein said single detector detects said transmission through said chambers simultaneously at wavelengths characteristic of said first isotopic component of said gas, and of said second isotopic component of said gas.

4. An isotopic gas analyzer according to claim 2, and wherein said at least one wavelength-stable source of radiation comprises at least two wavelength-stable sources of radiation, each of wavelengths characteristic of one isotopic component of said gas.

5. An isotopic gas analyzer according to claim 4, said at least two wavelength-stable sources of radiation operating with respective at least first and second different timing characteristics, said analyzer also comprising a detection differentiator receiving an output from said at least one detector and distinguishing outputs corresponding to said at least two wavelength-stable sources on the basis of said first and second different timing characteristics.

6. An isotopic gas analyzer according to claim 5 and also comprising a second detector, wherein said single detector views absorption signal outputs from said at least two wavelength-stable sources and said second detector views zero calibration signals from said at least two wavelength-stable sources.

7. An isotopic gas analyzer according to claim 2, and wherein said at least one wavelength-stable source of radiation is one wavelength-stable source of wavelengths characteristic of at least said first and second isotopic components.

8. An isotopic gas analyzer according to claim 2, and wherein said at least one wavelength-stable source is at least one gas discharge lamp.

9. An isotopic gas analyzer according to claim 2 and wherein at least one of the environmental conditions of at least one of said samples of said gas and said reference gas are the same.

10. An isotopic gas analyzer according to claim 2 and also comprising osmotic means for achieving substantially the same isotopic concentration in said sample of gas and said reference gas.

11. An isotopic gas analyzer according to claim 2 and also comprising pumping means for achieving substantially the same isotopic concentration in said sample of gas and said reference gas.

12. An isotopic gas analyzer according to claim 2, and also comprising mechanical means for changing the length of at least one of said sample chambers and reference chambers for achieving substantially the same isotopic absorption in said sample of gas and said reference gas.

13. An isotopic gas analyzer according to claim 2 and wherein said analyzed gas is exhaled breath.

14. An isotopic gas analyzer according to claim 2 and also comprising a beam homogenizer to compensate for inhomogeneity in an optical path.

15. An isotopic gas analyzer according to claim 2 and wherein the spectral ranges of said at least first and second isotopic components are non-overlapping.

16. An isotopic gas analyzer according to claim 2 and wherein said sample chambers are interconnected pneumatically.

17. An isotopic gas analyzer according to claim 2 and wherein said reference chambers are interconnected pneumatically.

18. An isotopic gas analyzer according to claim 2 and wherein said sample chambers are connected thermally.

19. An isotopic gas analyzer according to claim 2 and wherein said reference chambers are connected thermally.

20. An isotopic gas analyzer according to claim 2 and wherein said first and said second isotopic components of said gas have a region of spectral overlap, and also comprising a system for lowering the pressure of said sample of gas, such that an absorption measurement in said region of spectral overlap is performed under conditions of lowered sample gas pressure.

21. An isotopic gas analyzer according to claim 1, and wherein said at least one detector detects said transmission through said chambers simultaneously at wavelengths characteristic of said first isotopic component of said gas, and of said second isotopic component of said gas.

22. An isotopic gas analyzer according to claim 1, and wherein said at least one wavelength-stable source of radiation comprises at least two wavelength-stable sources of radiation, each of wavelengths characteristic of one isotopic component of said gas.

23. An isotopic gas analyzer according to claim 22, and wherein said at least two wavelength-stable sources are gas discharge lamps.

24. An isotopic gas analyzer according to claim 22 and wherein said at least two wavelength-stable sources of radiation operate with respective at least first and second different timing characteristics, said analyzer also comprising a detection differentiator receiving an output from said at least one detector and distinguishing outputs corresponding to said at least at least two wavelength-stable sources on the basis of said first and second different timing characteristics.

25. An isotopic gas analyzer according to claim 24 and wherein said at least first and second different timing characteristics are first and second frequencies.

26. An isotopic gas analyzer according to claim 24 and wherein said at least first and second different timing characteristics are first and second phases.

27. An isotopic gas analyzer according to claim 24 and wherein said at least one detector is a single detector viewing outputs of said at least two wavelength-stable sources.

28. An isotopic gas analyzer according to claim 24 and wherein said at least one detector comprises two detectors, each viewing one of said at least two wavelength-stable sources of radiation.

29. An isotopic gas analyzer according to claim 24 and wherein said at least one detector comprises two detectors, and wherein one of said two detectors views absorption signal outputs from said at least two wavelength-stable sources and the other of said two detectors views zero calibration signals from said at least two wavelength-stable sources.

30. An isotopic gas analyzer according to claim 24 and wherein said detection differentiator comprises first and second synchronized signal processors.

31. An isotopic gas analyzer according to claim 1, and wherein said at least one wavelength-stable source of radiation is one wavelength-stable source of wavelengths characteristic of at least said first and second isotopic components.

32. An isotopic gas analyzer according to claim 31, and wherein said at least one wavelength-stable source is at least one gas discharge lamp.

33. An isotopic gas analyzer according to claim 1, and wherein said at least one wavelength-stable source is at least one gas discharge lamp.

34. An isotopic gas analyzer according to claim 33, and wherein said at least one gas discharge lamp is a single gas discharge lamp containing at least a first and a second excitation gas.

35. An isotopic gas analyzer according to claim 34, and also comprising:

at least first and second filters, each corresponding to a part of respective first and second spectra of said at least first and second excitation gas, said filters being interposed between said at least one wavelength-stable source of radiation and said at least one detector; and

a detection differentiator cooperating with said detector for distinguishing detector outputs corresponding to said first and second spectra.

36. An isotopic gas analyzer according to claim 35, and wherein said detection differentiator comprises at least one light valve modulating light passing through at least one of said first and second filters, in accordance with a known timing sequence.

37. An isotopic gas analyzer according to claim 36 and wherein said light valve is a chopper.

38. An isotopic gas analyzer according to claim 36 and wherein said light valve is a spatial light modulator.

39. An isotopic gas analyzer according to claim 36 and wherein said at least one light valve is operated with respective first and second different timing characteristics, and wherein said detection differentiator also comprises a detector output discriminator receiving an output from said detector and distinguishing outputs corresponding to said first and second excitation gases on the basis of said first and second different timing characteristics.

40. An isotopic gas analyzer according to claim 39 and wherein said first and second different timing characteristics are first and second frequencies.

41. An isotopic gas analyzer according to claim 40 and wherein said first and second different timing characteristics are first and second phases.

42. An isotopic gas analyzer according to claim 40 and wherein said detection differentiator comprises first and second synchronized signal processors.

43. An isotopic gas analyzer according to claim 36, and also comprising a zero calibration channel, and wherein said light modulation is also operative to modulate light transmitted through at least one of said sample chambers, reference chambers and said zero calibration channel.

44. An isotopic gas analyzer according to claim 34, and wherein said first and second excitation gases are isotopically labeled.

45. An isotopic gas analyzer according to claim 34, and wherein said filters are at least one of optical and gaseous filters.

46. An isotopic gas analyzer according to claim 34, and wherein said at least first and second excitation gases having overlapping spectral ranges including at least some interdigitated spectral lines;

and also comprising a gas contents indicator receiving an output from said at least one detector and employing information detected by said at least one detector from at least two of said at least some interdigitated spectral lines.

47. An isotopic gas analyzer according to claim 46 and wherein said gas to be analyzed is maintained at a pressure below atmospheric pressure.

48. An isotopic gas analyzer according to claim 46 and also comprising filters to isolate non overlapping spectral ranges including at least some interdigitated spectral lines.

49. An isotopic gas analyzer according to claim 33, and wherein said at least one gas discharge lamp is two gas discharge lamps, containing respectively a first and a second excitation gas.

50. An isotopic gas analyzer according to claim 1, and wherein said at least one wavelength-stable source comprises at least one filter to define said wavelengths characteristic of at least one of said first and second isotopic components.

51. An isotopic gas analyzer according to claim 1 and wherein at least one of the environmental conditions of at least one of said samples of said gas and said reference gas are the same.

52. An isotopic gas analyzer according to claim 51 and wherein said reference gas comprises at least part of a sample of said gas.

53. An isotopic gas analyzer according to claim 51 and wherein said reference gas is a mixture containing at least one of said isotopic components at a known pressure and concentration.

54. An isotopic gas analyzer according to claim 1 and wherein said first sample of said gas and said second sample of said gas have essentially the same composition.

55. An isotopic gas analyzer according to claim 1 and also comprising osmotic means for achieving substantially the same isotopic concentration in said sample of gas and said reference gas.

56. An isotopic gas analyzer according to claim 1 and also comprising pumping means for achieving substantially the same isotopic concentration in said sample of gas and said reference gas.

57. An isotopic gas analyzer according to claim 1, and also comprising mechanical means for changing the length of at least one of said sample chambers and reference chambers for achieving substantially the same isotopic absorption in said sample of gas and said reference gas.

58. An isotopic gas analyzer according to claim 1 and wherein said analyzed gas is exhaled breath.

59. An isotopic gas analyzer according to claim 58 and wherein said exhaled breath is continuously sampled by use of a connecting nasal cannula.

60. An isotopic gas analyzer according to claim 58 and wherein said exhaled breath is continuously sampled by use of a breathing tube.

61. An isotopic gas analyzer according to claim 58 and wherein only a selected part of said exhaled breath is used for said analyzing.

62. An isotopic gas analyzer according to claim 1 and also comprising a beam homogenizer to compensate for inhomogeneity in an optical path.

63. An isotopic gas analyzer according to claim 1 and wherein the spectral ranges of said at least first and second isotopic components are non-overlapping.

64. An isotopic gas analyzer according to claim 1 and also comprising a zero calibration channel and an array of detectors, said array of detectors monitoring at least one of said sample chamber, said reference chamber and said zero calibration channel.

65. An isotopic gas analyzer according to claim 1 and wherein said sample chambers are interconnected pneumatically.

66. An isotopic gas analyzer according to claim 1 and wherein said reference chambers are interconnected pneumatically.

67. An isotopic gas analyzer according to claim 1 and wherein said sample chambers are connected thermally.

68. An isotopic gas analyzer according to claim 1 and wherein said reference chambers are connected thermally.

69. An isotopic gas analyzer according to claim 1 and wherein said first and said second isotopic components of said gas have a region of spectral overlap, and also comprising a system for lowering the pressure of said sample of gas, such that an absorption measurement in said region of spectral overlap is performed under conditions of lowered sample gas pressure.