Binding protein as biosensors

• US 7,064,103 B2
• Filed: 01/04/2002
• Issued: 06/20/2006
• Est. Priority Date: 01/04/2002
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Pitner, J. Bruce, Gestwicki, Jason E., Hsieh, Helen V., Pfieffer, Zachary A.
• Primary Examiner(s): Campell, Bruce R.
• Assistant Examiner(s): TELLER, ROY R
• Application Number:
  US10/039,799
• Publication Number:
  US 20030130167A1
• Time in Patent Office: 1,628 Days
• Field of Search: 514/2, 435/14, 435/23, 435/25, 536/1.11
• US Class Current: 435/14
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

The invention is directed to compositions of mutated binding proteins containing thiol groups for coupling to sensor surfaces, analyte biosensor devices derived there from, and methods of their use as analyte biosensors both in vitro and in vivo.

Claims

1. A biosensor comprising:

a) at least one mutated glucose/galactose binding protein having at least one substituted or added cysteine group; and

b) at least one sensor surface wherein said mutated binding protein is coupled through said cysteine group to said surface;

wherein said at least one sensor surface provides a detectable signal resulting from a change in refractive index when said mutated binding protein binds to analyte.

2. The biosensor of claim 1 wherein said analyte is glucose or galactose.

3. The biosensor of claim 1 wherein said mutated glucose/galactose binding protein has one amino acid substitution.

4. The biosensor of claim 1 wherein said mutated glucose/galactose binding protein has at least two amino acid substitutions.

5. The biosensor of claim 1 wherein said mutated glucose binding protein includes one amino acid substitution selected from the group consisting of a cysteine at position 11, a cysteine at position 14, a cysteine at position 19, a cysteine at position 43, a cysteine at position 74, a cysteine at position 107, a cysteine at position 110, a cysteine at position 112, a cysteine at position 113, ∥

, ∥

cysteine at position 149, a cysteine at position 213, a cysteine at position 216, a cysteine at position 238, a cysteine at position 287, and a cysteine at position 292.

6. The biosensor of claim 3 wherein said mutated binding protein has at least one histidine tag.

7. The biosensor of claim 1 wherein said mutated glucose/galactose binding protein includes a cysteine present at position 213.

9. The biosensor of claim 1 wherein said mutated glucose/galactose binding protein includes a cysteine present at position 149 coupled to said sensor surface.

10. The biosensor of claim 9 wherein said mutated glucose/galactose binding protein further includes a histidine tag.

11. The biosensor of claim 1 wherein said mutated glucose/galactose binding protein includes at least two amino acid substitutions selected from the group consisting of:

a cysteine at position 112 and a serine at position 238, a cysteine at position 149 and a serine at position 238, a cysteine at position 152 and a cysteine at position 182, a cysteine at position 152 and a serine at position 213, a cysteine at position 213 and a cysteine at position 238, a cysteine at position 149 and an arginine at position 213, a cysteine at position 149 and a serine at position 213 and a serine at position 238, and a cysteine at position 149 and an arginine at position 213 and a serine at position 238 coupled to said sensor surface.

12. The biosensor of claim 11 wherein said mutated glucose/galactose binding protein further includes a histidine tag.

8. The biosensor of 7 wherein said mutated glucose/galactose binding protein further includes a histidine tag.