Bioartificial device for the storage, cultivation and/or multiplication of cells

• US 7,067,307 B2
• Filed: 08/14/2003
• Issued: 06/27/2006
• Est. Priority Date: 02/14/2001
• Status: Expired due to Fees

• Current Assignee: Raimung Margreiter
• Original Assignee: Raimung Margreiter
• Inventors: Hengster, Paul, Hochleitner, Boris-Wolfgang
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US10/641,275
• Publication Number:
  US 20040033593A1
• Time in Patent Office: 1,048 Days
• Field of Search: 435/297.2, 435/297.3, 435/297.5, 435/298.2, 435/295.3, 422/48, 210/646, 210/321.64, 210/321.68, 210/321.67, 210/321.74, 210/321.78, 210/321.8
• US Class Current: 435/297.2
• CPC Class Codes: A61M 1/3472 with treatment of the filtrate
• CPC Class Codes: A61M 1/3489 by biological cells, e.g. bioreactor
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: C12M 21/08 for producing artificial tissue or for ex-vivo cultivation of tissue prostheses A61F2/00, grafts A61L27/00
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis

Abstract

The bioartificial reactor has a reactor vessel. A cell culture chamber in the reactor, in particular for liver cells, is divided off from a flow chamber for a nutritive medium and from a flow chamber for plasma and blood by semipermeable walls. At least one flow chamber is formed as a tube that is helically wound in the shape of a screw and that is made of semipermeable material.

Claims

1. A bioartificial device, comprising:

a reactor vessel having a first end and a second opposite end, said reactor vessel being formed with a cell culture chamber, a first throughflow chamber for a nutrient medium separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, and a second throughflow chamber for a body substance separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, said first throughflow chamber for the nutrient medium being centrally disposed and being cylindrical with a wall including a semi-permeable membrane, and said second throughflow chamber being formed by a tube of semi-permeable material helically disposed in said cell culture chamber;

said inlet ends of said first and second throughflow chambers being in fluid communication with feed connections disposed on said first end of said reactor vessel and said outlet ends of said first and second throughflow chambers being in fluid communication with discharge connections disposed on said first end of said reactor vessel with tubes guided through said reactor vessel and extending from said discharge connections to said outlet ends of said throughflow chambers.

2. The device according to claim 1, wherein said rector vessel is formed with an outer wall, said second throughflow chamber is a tube coil arranged near said outer wall, and said first throughflow chamber is disposed centrally in said reactor vessel inside said tube coil.

3. The device according to claim 1, wherein said semi-permeable membrane is supported on a wire mesh.

4. The device according to claim 1, wherein at least one of said first and second throughflow chambers is supported on a driven carrier.

5. The device according to claim 4, which comprises a central drive shaft connected to said driven carrier for said throughflow chamber for the nutrient medium.

6. The device according to claim 4, wherein said reactor vessel is formed with a driven flange connected to said driven carrier of said tube coil through which the body substance flows, and said driven flange is rotatably supported on a central drive shaft.

7. The device according to claim 1, wherein each of said first and second throughflow chambers is supported on a driven carrier and said driven carriers have mutually different rotational speeds.

8. The device according to claim 1, wherein said reactor vessel is formed with a driven peripheral wall and each said throughflow chamber is disposed on a stationary support.

9. The device according to claim 8, which comprises at least one stirring element assigned to said driven peripheral wall and projecting between said first and second throughflow chambers.

10. The device according to claim 9, wherein said reactor vessel has a driven casing with a base, and said stirring element rises from said base.

11. The device according to claim 1, wherein said reactor vessel is a cylindrical vessel with a substantially horizontal axis.

12. The device according to claim 1, wherein said cell culture chamber is allocated at least one inspection and/or pressure-equalization opening.

13. The device according to claim 1, wherein said cell culture chamber is configured for liver cells.

14. The device according to claim 1, wherein said second throughflow chamber is configured for a body substance selected from the group consisting of plasma and blood.

15. A bioartificial device, comprising:

a reactor vessel having a first end and a second opposite end, said reactor vessel being formed with a cell culture chamber, a first throughflow chamber for a nutrient medium separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, and a second throughflow chamber for a body substance separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, at least one of said first and second throughflow chambers being formed by a tube of semi-permeable material helically disposed in said cell culture chamber and at least one of said first and second throughflow chambers being supported on a driven carrier;

said inlet ends of said first and second throughflow chambers being in fluid communication with feed connections disposed on said first end of said reactor vessel and said outlet ends of said first and second throughflow chambers being in fluid communication with discharge connections disposed on said first end of said reactor vessel with tubes guided through said reactor vessel and extending from said discharge connections to said outlet ends of said throughflow chambers.

16. The device according to claim 15, wherein said rector vessel is formed with an outer wall, said second throughflow chamber is a tube coil arranged near said outer wall, and said first throughflow chamber is disposed centrally in said reactor vessel inside said tube coil.

17. The device according to claim 15, wherein each of said first and second throughflow chambers is formed by a tube of semi-permeable material arranged helically in said cell culture chamber, and said two tube coils are disposed coaxially.

18. The device according to claim 15, which comprises a central drive shaft connected to said driven carrier for said throughflow chamber for the nutrient medium.

19. The device according to claim 15, wherein said reactor vessel is formed with a driven flange connected to said driven carrier of said tube coil through which the body substance flows, and said driven flange is rotatably supported on a central drive shaft.

20. The device according to claim 15, wherein each of said first and second throughflow chambers is supported on a driven carrier and said driven carriers have mutually different rotational speeds.

21. The device according to claim 15, wherein said reactor vessel is formed with a driven peripheral wall and each said throughflow chamber is disposed on a stationary support.

22. The device according to claim 21, which comprises at least one stirring element assigned to said driven peripheral wall and projecting between said first and second throughflow chambers.

23. The device according to claim 22, wherein said reactor vessel has a driven casing with a base, and said stirring element rises from said base.

24. The device according to claim 15, wherein said reactor vessel is a cylindrical vessel with a substantially horizontal axis.

25. The device according to claim 15, wherein said cell culture chamber is allocated at least one inspection and/or pressure-equalization opening.

26. The device according to claim 15, wherein said cell culture chamber is configured for liver cells.

27. The device according to claim 15, wherein said second throughflow chamber is configured for a body substance selected from the group consisting of plasma and blood.

28. A bioartificial device, comprising:

a reactor vessel having a first end and a second opposite end, said reactor vessel being formed with a cell culture chamber, a first throughflow chamber for a nutrient medium separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, and a second throughflow chamber for a body substance separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel at least one of said first and second throughflow chambers being formed by a tube of semi-permeable material helically disposed in said cell culture chamber, said reactor vessel being formed with a driven peripheral wall, and each said throughflow chamber being disposed on a stationary support;

said inlet ends of said first and second throughflow chambers being in fluid communication with feed connections disposed on said first end of said reactor vessel and said outlet ends of said first and second throughflow chambers being in fluid communication with discharge connections disposed on said first end of said reactor vessel with tubes guided through said reactor vessel and extending from said discharge connections to said outlet ends of said throughflow chambers.

29. The device according to claim 28, wherein said rector vessel is formed with an outer wall, said second throughflow chamber is a tube coil arranged near said outer wall, and said first throughflow chamber is disposed centrally in said reactor vessel inside said tube coil.

30. The device according to claim 28, wherein each of said first and second throughflow chambers is formed by a tube of semi-permeable material arranged helically in said cell culture chamber, and said two tube coils are disposed coaxially.

31. The device according to claim 28, which comprises at least one stirring element assigned to said driven peripheral wall and projecting between said first and second throughflow chambers.

32. The device according to claim 31, wherein said reactor vessel has a driven casing with a base, and said stirring element rises from said base.

33. The device according to claim 28, wherein said reactor vessel is a cylindrical vessel with a substantially horizontal axis.

34. The device according to claim 28, wherein said cell culture chamber is allocated at least one inspection and/or pressure-equalization opening.

35. The device according to claim 28, wherein said cell culture chamber is configured for liver cells.

36. The device according to claim 28, wherein said second throughflow chamber is configured for a body substance selected from the group consisting of plasma and blood.

37. A bioartificial device, comprising:

a reactor vessel having a first end and a second opposite end, said reactor vessel being formed with a cell culture chamber, a first throughflow chamber for a nutrient medium separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, and a second throughflow chamber for a body substance separated from said cell culture chamber by a semipermeable wall and including an inlet end adjacent said first end of said reactor vessel and an outlet end adjacent said second end of said reactor vessel, at least one of said first and second throughflow chambers being formed by a tube of semi-permeable material helically disposed in said cell culture chamber, said inlet ends of said first and second throughflow chambers being in fluid communication with feed connections disposed on said first end of said reactor vessel and said outlet ends of said first and second throughflow chambers being in fluid communication with discharge connections disposed on said first end of said reactor vessel with tubes guided through said reactor vessel and extending from said discharge connections to said outlet ends of said throughflow chambers.

38. The device according to claim 37, wherein said rector vessel is formed with an outer wall, said second throughflow chamber is a tube coil arranged near said outer wall, and said first throughflow chamber is disposed centrally in said reactor vessel inside said tube coil.

39. The device according to claim 37, wherein each of said first and second throughflow chambers is formed by a tube of semi-permeable material arranged helically in said cell culture chamber, and said two tube coils are disposed coaxially.

40. The device according to claim 37, wherein at least one of said first and second throughflow chambers is supported on a driven carrier.

41. The device according to claim 40, which comprises a central drive shaft connected to said driven carrier for said throughflow chamber for the nutrient medium.

42. The device according to claim 40, wherein said reactor vessel is formed with a driven flange connected to said driven carrier of said tube coil through which the body substance flows, and said driven flange is rotatably supported on a central drive shaft.

43. The device according to claim 37, wherein each of said first and second throughflow chambers is supported on a driven carrier and said driven carriers have mutually different rotational speeds.

44. The device according to claim 37, wherein said reactor vessel is formed with a driven peripheral wall and each said throughflow chamber is disposed on a stationary support.

45. The device according to claim 44, which comprises at least one stirring element assigned to said driven peripheral wall and projecting between said first and second throughflow chambers.

46. The device according to claim 45, wherein said reactor vessel has a driven casing with a base, and said stirring element rises from said base.

47. The device according to claim 37, wherein said reactor vessel is a cylindrical vessel with a substantially horizontal axis.

48. The device according to claim 37, wherein said cell culture chamber is allocated at least one inspection and/or pressure-equalization opening.

49. The device according to claim 37, wherein said cell culture chamber is configured for liver cells.

50. The device according to claim 37, wherein said second throughflow chamber is configured for a body substance selected from the group consisting of plasma and blood.