Sensor for luminescense-optical determination of an analyte
First Claim
1. An optical chemical sensor functioning in accordance with the FRET principle for detecting an analyte in a sample medium comprising:
- a donor phase comprising a first luminophore and having an emission spectrum; and
an acceptor phase comprising a chromophore or a second luminophore responsive to the analyte and having an absorption spectrum, whereinthe donor phase is essentially impermeable to the sample medium or components of the medium capable of affecting the luminescence characteristics of the first luminophore,there is at least partial overlap between the emission spectrum of the donor phase and the absorption spectrum of the acceptor phase, andthe acceptor phase is in sufficient spatial proximity to the donor phase for nonradiative energy transfer.
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Abstract
The invention relates to an optochemical sensor functioning in accordance with the FRET-principle and exhibiting an acceptor (chromophore or luminophore) responsive to an analyte contained in a sample medium as well as a donor (luminophore), characterized in that acceptor and donor are located in separate chemical phases, whereby the phase containing the donor is essentially impermeable to the sample medium or to components of the sample medium affecting the luminescence characteristics of the luminophore. The invention further relates to a method for qualitative and/or quantitative determination of at least one analyte and/or component of a gaseous or liquid measuring medium according to the FRET-principle, characterized by the use of the sensor according to the invention.
29 Citations
28 Claims
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1. An optical chemical sensor functioning in accordance with the FRET principle for detecting an analyte in a sample medium comprising:
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a donor phase comprising a first luminophore and having an emission spectrum; and an acceptor phase comprising a chromophore or a second luminophore responsive to the analyte and having an absorption spectrum, wherein the donor phase is essentially impermeable to the sample medium or components of the medium capable of affecting the luminescence characteristics of the first luminophore, there is at least partial overlap between the emission spectrum of the donor phase and the absorption spectrum of the acceptor phase, and the acceptor phase is in sufficient spatial proximity to the donor phase for nonradiative energy transfer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification