Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids

• US 7,067,722 B2
• Filed: 06/21/2002
• Issued: 06/27/2006
• Est. Priority Date: 08/26/1999
• Status: Expired due to Fees

• Current Assignee: Monsanto Technology LLC (Bayer AG)
• Original Assignee: Monsanto Technology LLC (Bayer AG)
• Inventors: Fillatti, JoAnne J.
• Primary Examiner(s): McElwain, Elizabeth F.
• Application Number:
  US10/176,149
• Publication Number:
  US 20030172399A1
• Time in Patent Office: 1,467 Days
• Field of Search: 800/281, 800/312
• US Class Current: 800/312
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8247 involving modified lipid metabolism, e.g. seed oil composition
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)

Abstract

The present invention is directed to nucleic acid molecules and nucleic acid constructs, and other agents associated with fatty acid synthesis, particularly the ratios of saturated and unsaturated fats. Moreover, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of saturated and unsaturated fats. In particular, the present invention is directed to plants incorporating such agents where the plants exhibit altered ratios of monounsaturated to polyunsaturated fatty acids.

Claims

1. A transformed soybean plant having a nucleic acid molecule that comprises

(a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 90% or greater identity to SEQ ID NO:

1, its complement and fragments of either, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad2-1, and

(b) a second nucleic acid molecule with a second nucleic acid sequence that has 90% or greater identity to SEQ ID NO;

14, its complement and fragments of either, wherein the second nucleic acid molecule is operable linked to the first promoter or a second promoter, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad3-1.

2. The transformed soybean plant according to claim 1, wherein a single promoter is operably linked to the first and second nucleic acid molecules.

3. The transformed soybean plant according to claim 2, wherein the single promoter is a seed specific promoter.

4. The transformed soybean plant according to claim 1, wherein the first promoter and the second promoter are both seed specific promoters.

5. The transformed soybean plant according to claim 1, wherein the first promoter and the second promoter are the same.

6. The transformed soybean plant according to claim 5, wherein the first promoter and the second promoter are both 7S promoters.

7. The transformed soybean plant according to claim 1, wherein the first promoter is different from the second promoter.

8. The transformed soybean plant according to claim 7, wherein the first promoter is a 7S promoter and the second promoter is a napin promoter.

9. The transformed soybean plant according to claim 1, wherein said first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a FAD2-1 gene while leaving the level of a transcript encoded by a FAD2-2 gene partially unaffected.

10. The transformed soybean plant according to claim 1, wherein said first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a FAD2-1 gene while leaving the level of a transcript encoded by a FAD2-2 gene substantially unaffected.

11. The transformed soybean plant according to claim 2, wherein said first nucleic acid molecule is transcribed and is capable of selectively reducing the level of a transcript encoded by a FAD2-1 gene while leaving the level of a transcript encoded by a FAD2-2 gene essentially unaffected.

12. A method of producing a soybean plant having a seed with reduced linolenic acid content comprising:

transforming a soybean plant with a nucleic acid molecule that comprises

(a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 90% or greater identity to SEQ ID NO;

1 and its complement, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad2-1,and

(b) a second nucleic acid molecule having a second nucleic acid sequence that has 90% or greater identity to SEQ ID NO;

14 and its complement, wherein the second nucleic. acid molecule is operably linked to the first promoter or a second promoter and capable of suppressing endogenous expression of Fad3-1; and

growing said plant, wherein said plant produces seed with less linolenic acid than a plant having a similar genetic background but lacking said nucleic acid molecule.

13. A method of producing a soybean plant having a seed with increased oleic acid content comprising:

transforming a soybean plant with a nucleic acid molecule that comprises

(a) a first promoter operably linked to a first nucleic acid molecule having a first nucleic acid sequence that has 90% or greater identity to SEQ ID NO;

1 and complement thereof, wherein said first nucleic acid molecule is capable of suppressing endogenous expression of Fad2-1, and

(b) a second nucleic acid molecule having a second nucleic acid sequence that has 90% or greater identity to SEQ ID NO;

14 and complements thereof, wherein the second nucleic acid molecule is operably linked to the first promoter or a second promoter and capable of suppressing endogenous expression of Fad3-1; and

growing said plant, wherein said plant produces seed with more oleic acid than a plant having similar genetic background but lacking said nucleic acid molecule.