Microfluidic sorting device

• US 7,068,874 B2
• Filed: 05/18/2004
• Issued: 06/27/2006
• Est. Priority Date: 11/28/2000
• Status: Expired due to Term

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Wang, Mark, Esener, Sadik C., Ata, Erhan Polatkan
• Primary Examiner(s): Ullah, Akm Enayet
• Application Number:
  US10/848,972
• Publication Number:
  US 20050164158A1
• Time in Patent Office: 770 Days
• Field of Search: 385 14- 19, 385/147, 250/251, 250/432.R
• US Class Current: 385/16
• CPC Class Codes: H05H 3/04 Acceleration by electromagnetic wave pressure
• CPC Class Codes: Y10T 428/24744 Longitudinal or transverse tubular cavity or cell

Abstract

Small particles, for example 5 μm diameter microspheres or cells, within, and moving with, a fluid, normally water, that is flowing within microfluidic channels within a radiation-transparent substrate, typically molded PDMS clear plastic, are selectively manipulated, normally by being pushed with optical pressure forces, with laser light, preferably as arises from VCSELs operating in Laguerre-Gaussian mode, at branching junctions in the microfluidic channels so as to enter into selected downstream branches, thereby realizing particle switching and sorting, including in parallel. Transport of the small particles thus transpires by microfluidics while manipulation in the manner of optical tweezers arises either from pushing due to optical scattering force, or from pulling due to an attractive optical gradient force. Whether pushed or pulled, the particles within the flowing fluid may be optically sensed, and highly-parallel, low-cost, cell- and particle-analysis devices efficiently realized, including as integrated on bio-chips.

Claims

1. A microfluidic sorting device comprising:

a substrate having a main microfluidic channel that branches into a plurality of microfluidic branch channels, the main microfluidic channel and the plurality of microfluidic branch channels adapted to contain a moving fluid having particles disposed therein; and

a light source that produces at least one light beam directed at the main microfluidic channel, the light beam selectively switching the particles into the plurality of microfluidic branch channels without optically trapping the particles.

2. The microfluidic sorting device of claim 1, wherein the particles comprise cells.

3. The microfluidic sorting device of claim 2, wherein the particles comprise live cells.

4. The microfluidic sorting device of claim 1, wherein the particles comprise biological samples.

5. The microfluidic sorting device of claim 1, wherein the substrate includes a top surface and a bottom surface, the light beam being directed at the main microfluidic channel through one of the top surface and the bottom surface.

6. The microfluidic sorting device of claim 1, wherein the substrate includes one or more side surfaces, the light beam being directed at the main microfluidic channel through one of the side surfaces.

7. The microfluidic sorting device of claim 1, wherein the substrate includes a microlens disposed therein to guide the at least one light beam.

8. The microfluidic sorting device of claim 1, wherein the substrate includes an optical waveguide disposed therein.

9. The microfluidic sorting device of claim 1, wherein the at least one light beam directed at the main microfluidic channel is stationary.

10. The microfluidic sorting device of claim 1, wherein the at least one light beam directed at the main microfluidic channel is translated relative to the substrate.

11. The microfluidic sorting device of claim 1, wherein the light source comprises a laser.

12. The microfluidic sorting device of claim 1, wherein the light source comprises a Vertical Cavity Surface Emitting Laser (VCSEL).

13. The microfluidic sorting device of claim 1 further comprising,

at least one of the plurality of microfluidic branch channels branching further into a plurality of sub-branch channels, and

an additional light source that produces at least one additional light beam directed at the at least one branch channel, the additional light beam selectively switching the particles into the plurality of sub-branch channels with non-trapping radiation pressure.

14. A microfluidic sorting device, comprising:

a main microfluidic channel to conduct a moving fluid flow comprising particles;

at least one branching junction in the main microfluidic channel;

a plurality of microfluidic branch channels connected to the at least one branching junction to branch at least a portion of the moving fluid flow into a plurality of branch moving fluid flows respectively in the microfluidic branch channels; and

at least one control module that directs at least one light beam at the main microfluidic channel to optically switch particles in the moving fluid flow into at least one of the microfluidic branch channels without optical trapping.

15. The device as in claim 14, further comprising a flow inducer to cause fluid flow in the main microfluidic channel and the microfluidic branch channels.

16. The device as in claim 14, wherein the at least one control module directs the at least one light beam perpendicular to a plane formed by at least two of the microfluidic branch channels.

17. The device as in claim 14, wherein the at least one control module directs the at least one light beam within a plane formed by at least two of the microfluidic branch channels.

18. The device as in claim 14, further comprising at least one lens to direct the at least one light beam to the main microfluidic channel.

19. The device as in claim 18, further comprising a substrate on which the main microfluidic channel and the microfluidic branch channels are formed, wherein the lens is a microlens fabricated in the substrate.

20. The device as in claim 14, further comprising at least one wave guide to direct the at least one light beam to the main microfluidic channel.

21. The device as in claim 20, further comprising a substrate on which the main microfluidic channel and the microfluidic branch channels are formed, wherein the wave guide is fabricated in the substrate.

22. The device as in claim 14, further comprising a mechanism to further sort sorted particles in one of the microfluidic branch channels.

23. The device as in claim 14, further comprising a mechanism to collect sorted particles from one of the microfluidic branch channels.

24. The device as in claim 14, further comprising a detection mechanism located upstream in the main microfluidic channel from a location where the at least one light beam intercepts with the main microfluidic channel.

25. The device as in claim 14, wherein the at least one control module is configured to use at least one light beam to translate a position of the selected particle to direct the selected particle in the moving fluid flow into the at least one of the microfluidic branch channels.

26. The device as in claim 25, wherein the at least one control module comprises a micro-mirror device which operates to translate a position of the selected particle.

27. The device as in claim 14, wherein the at least one control module is configured to use the at least one light beam to optically push a selected particle in the moving fluid flow into the at least one of the microfluidic branch channels without optically trapping the selected particle.

28. The device as in claim 14, wherein the at least one control module is configured to use the at least one light beam to optically pull a selected particle in the moving fluid flow into the at least one of the microfluidic branch channels without optically trapping the selected particle.

29. The device as in claim 14, further comprising a sensing mechanism to optically sense particles in the main microfluidic channel, and wherein the at least one control module acts on a sensing result of the sensing mechanism to select and optically switch the particles in the main microfluidic channel.

30. The device as in claim 14, wherein the at least one control module operates to select a particle according to an emission wavelength of the particle.

31. The device as in claim 14, wherein the at least one control module comprises a stimulation mechanism to optically stimulate emission from the particles in the main microfluidic channel, and a sensing mechanism to sense fluorescent light emitted by optically stimulated particles.

32. The device as in claim 31, wherein the at least one control module acts on the sensed fluorescent light to optically switch the particles in the main microfluidic channel.

33. A method for optically sorting particles in a flowing fluid, comprising:

supplying a flowing fluid comprising particles to a main microfluidic channel that branches at at least one junction into at least two branch microfluidic channels; and

using at least one optical beam to optically switch particles in the main microfluidic channel into at least one of the at least two branch microfluidic channels without optical trapping.

34. The method as in claim 33, further comprising using the at least one optical beam to optically switch cells in the main microfluidic channel.

35. The method as in claim 34, wherein the cells in the main microfluidic channel comprise live cells.

36. The method as in claim 33, further comprising using the at least one optical beam to optically switch biological samples in the main microfluidic channel.

37. The method as in claim 33, further comprising directing the optical beam in a direction substantially perpendicular to a plane formed by the at least two microfluidic branch channels.

38. The method as in claim 33, further comprising directing the optical beam in a direction substantially parallel to a plane formed by the at least two microfluidic branch channels.

39. The method as in claim 33, further comprising collecting sorted particles from a branch microfluidic channel.

40. The method as in claim 33, comprising further sorting sorted particles in a branch microfluidic channel.

41. The method as in claim 33, further comprising optically sensing particles in the main microfluidic channel, and using a sensing result from the optical sensing to select and optically switch particles in the main microfluidic channel into at least one of the at least two branch microfluidic channels.

42. The method as in claim 41, wherein the optical sensing comprises optically stimulating the particles and subsequently sensing emission from stimulated particles.

43. The method as in claim 40, further comprising using an emission wavelength of the particles to select particles.

44. The method as in claim 33, wherein the at least one optical beam is translated relative to the main microfluidic channel and the branch microfluidic channels.

45. The method as in claim 33, wherein a substrate on which the main microfluidic channel and the at least two branch microfluidic channels are formed is translated relative to the at least one optical beam.

46. The method as in claim 33, further comprising using the at least one optical beam to push a particle without optical trapping of the particle when switching the particle into one of the at least two branch microfluidic channels.

47. The method as in claim 33, further comprising using the at least one optical beam to pull a particle without optical trapping of the particle when switching the particle into one of the at least two branch microfluidic channels.

48. The method as in claim 33, further comprising using the at least one optical beam to optically switch a cell among the particles without optical trapping of the cell when switching the cell into one of the at least two branch microfluidic channels.

49. The method as in claim 33, further comprising using the at least one optical beam to optically switch a live cell among the particles without optical trapping of the live cell when switching the live cell into one of the at least two branch microfluidic channels.

50. The method as in claim 33, further comprising using the at least one optical beam to optically switch a biological sample among the particles without optical trapping of the biological sample when switching the biological sample into one of the at least two branch microfluidic channels.