Methods and devices for detecting printhead misalignment of an in situ polymeric array synthesis device
First Claim
1. A method of detecting deposition unit misalignment of an in situ polymeric array synthesis device, said method comprising:
- synthesizing at least one test probe feature on a substrate using said in situ polymeric array synthesis device;
contacting said at least one test probe feature with at least two different distinguishably labeled targets; and
evaluating the binding of said two different distinguishably labeled targets to said at least one test probe feature to detect any pulse jet misalignment of said in situ polymeric array synthesis device.
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Accused Products
Abstract
Methods and devices for detecting deposition unit misalignment, e.g., printhead misalignment, of an in situ polymeric, e.g., a nucleic acid, array synthesis device are provided. In accordance with the subject methods, at least one test probe feature is synthesized on a substrate using an in situ polymeric array, e.g., nucleic acid array or protein array, synthesis device. The at least one test probe feature is then contacted with at least two different distinguishably labeled targets, e.g., target nucleic acids. The binding of the targets to the at least one test probe feature is then evaluated to detect any misalignment, e.g., deposition unit or pulse jet misalignments, of the synthesis device. Also provided are substrates having at least one test probe feature and at least one polymeric array, as well as methods of using the substrates in array assays. Also included are kits for use in practicing the subject methods.
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Citations
27 Claims
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1. A method of detecting deposition unit misalignment of an in situ polymeric array synthesis device, said method comprising:
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synthesizing at least one test probe feature on a substrate using said in situ polymeric array synthesis device; contacting said at least one test probe feature with at least two different distinguishably labeled targets; and evaluating the binding of said two different distinguishably labeled targets to said at least one test probe feature to detect any pulse jet misalignment of said in situ polymeric array synthesis device. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of detecting printhead misalignment of an in situ nucleic acid array synthesis device, said method comprising:
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synthesizing at least one test probe feature on a substrate using said in situ nucleic acid array synthesis device; contacting said at least one test probe feature with at least two different distinguishably labeled target nucleic acids; and evaluating the binding of said two different distinguishably labeled target nucleic acids to said at least one test probe feature to detect any printhead misalignment of said in situ nucleic acid array synthesis device. - View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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Specification