Method for assaying glycated protein
First Claim
1. A method for assaying the presence and/or amount of an alpha glycated peptide in a sample comprising:
- treating a sample suspected of containing an α
-glycated peptide with at least one oxidase which produces hydrogen peroxide upon reacting with the α
-glycated peptide, and determining the presence and/or amount of a generated product or consumed substance by said reaction.
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Abstract
Based on a principle that is different to that of a conventional enzymatic method, the present invention provides a novel method for assaying a glycated protein by a simple procedure, within a short period of time, and with high accuracy, and a reagent kit for assaying used in the method. The method for assaying a glycated protein in a sample is realized by treating a glycated protein-containing sample with protease to liberate a glycated peptide, preferably an α-glycated peptide, particularly preferably an α-glycated dipeptide, from a glycated protein, allowing an oxidase to react with the liberated glycated peptide, and determining the produced hydrogen peroxide.
38 Citations
28 Claims
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1. A method for assaying the presence and/or amount of an alpha glycated peptide in a sample comprising:
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treating a sample suspected of containing an α
-glycated peptide with at least one oxidase which produces hydrogen peroxide upon reacting with the α
-glycated peptide, anddetermining the presence and/or amount of a generated product or consumed substance by said reaction. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for assaying the presence and/or amount of a glycated protein in a sample comprising:
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treating a sample suspected of containing a glycated protein with at least one protease and determining the presence or absence of, and/or amount of, fructosyl valyl histidine liberated from said sample. - View Dependent Claims (22)
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23. A kit comprising:
an oxidase which produces hydrogen peroxide by reacting with an α
-glycated peptide.- View Dependent Claims (24, 25, 26, 27, 28)
Specification