Methodology and apparatus for the detection of biological substances

• US 7,075,428 B1
• Filed: 08/18/2005
• Issued: 07/11/2006
• Est. Priority Date: 11/20/2003
• Status: Expired due to Fees

• Current Assignee: Jeffrey L. Riggs, BioWarn LLC (KKR & Co., Inc.)
• Original Assignee: BioWarn LLC (KKR & Co., Inc.)
• Inventors: Oleynik, Vladislav A.
• Primary Examiner(s): Wu, Daniel
• Assistant Examiner(s): Blount, Eric
• Application Number:
  US11/206,200
• Time in Patent Office: 327 Days
• Field of Search: 340/539.26
• US Class Current: 340/539.26
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A methodology and an apparatus for the detection of biological substances employing the integration of multiple functions and units designed into and implemented in the form of an individual silicon chip, described as a sensor unit. The deployment of a set of sensor units as a group results in a distributed detecting, discriminating, and alerting network. Distribution of the sensor units facilitates the on-the-spot detection of different biological substances such as viruses, bacteria, spores, allergens, and other toxins that can be suspended in multiple media (air, liquid, blood, etc.). Besides detection/sensing, the individual sensor units perform: data acquisition, data development, data storage, statistical analysis, and data transmission. A set of sensor units deployed in proximity to each other can be designated as a group and act as a distributed sensing network with consistent and reliable data flow to a router and further to a central computer for extended data synthesis, analysis, and decision support. The group deployment facilitates achieving enhanced security and wider sensing capability.

Claims

1. A system for determining the existence of toxic biological substances, comprising:

a. a plurality of sensor units, each sensor unit further comprising;

i. a first ligand, oriented to provide optimal sensing capability;

ii. a first electrostatic pulse signature signal being generated by an interaction occurring when the first ligand binds to at least one first toxic biological target;

iii. an electrostatic sensing surface, positioned in proximity to the first ligand for detecting the first electrostatic pulse signature signal being generated;

iv. a measurement means for measuring the detected first electrostatic pulse signature signal provided in proximity with the sensing surface;

v. a processor provided with a memory having a plurality of stored electrostatic pulse signature signals controlling the operation of each of the sensor units;

vi. a comparison device connected between the measurement means and the processor for comparing the electrostatic pulse signature signal measured by the measurement means with the stored electrostatic pulse signature signals and generating comparison information;

vii. an antenna for transmitting and receiving the comparison information; and

b. a centralized station for controlling the operation of the plurality of sensor units, the centralized station including a central processor, a central memory and an antenna for transmitting and receiving comparison information from the plurality of sensor units, the central station generating an alert based upon the comparison information received from said plurality of sensor units.

2. The system in accordance with claim 1, wherein the alert is generated based upon the number and type of toxic biological substances sensed by the plurality of sensor units.

3. The system in accordance with claim 1, further comprising a router unit used to transmit information received from the plurality of sensor units to the centralized station, as well as to transmit information to the plurality of sensor units.

4. The system in accordance with claim 2, further comprising a router unit used to transmit information received from the plurality of sensor units to the centralized station, as well as to transmit information to said the plurality of sensor units.

5. The system in accordance with claim 3, wherein each of the plurality of sensor units transmits and receives data directly from one or more of the plurality of sensor units.

6. The system in accordance with claim 4, wherein each of the plurality of sensor units transmits and receives data directly from one or more of the plurality of sensor units.

7. The system in accordance with claim 1, further comprising:

a plurality of ligands, each of the ligands including a ligand sensing surface and ligand orienting surface opposite the ligand sensing surface;

the ligand sensing surface of the first ligand of the plurality of ligands completely binds with only the first biological target to produce the first electric pulse signature signal; and

the ligand sensing surface of a second ligand of a plurality of ligands completely binds with only a second biological target to produce a second electric pulse signature signal.

8. The system in accordance with claim 1, wherein the first ligand binds only with the first biological target wherein the interaction between the first ligand and the first biological target produces a first electrostatic pulse signature signal.

9. The system in accordance with claim 1, wherein the measurement means is a field effect transistor (FET) provided with a source region, a gate region and a drain region.

10. The system in accordance with claim 8, wherein the measurement means is a field effect transistor (FET) provided with a source region, a gate region and a drain region.

11. The system in accordance with claim 9, wherein the FET is an electron sensitive field effect transistor (ESFET).

12. The system in accordance with claim 8, further comprising a biological amplification unit connected to the first ligand.

13. The system in accordance with claim 10, further comprising the first ligand of a plurality of ligands, each of the plurality of ligands including a ligand sensing surface and at least one orienting surface, said orienting surface of at least a portion of the plurality of ligands provided in proximity to the gate region of the FET.

14. The system in accordance with claim 9, further comprising a plurality of ligands provided in proximity to the measurement means.

15. The system in accordance with claim 13, wherein the ligand sensing surface of the first ligand of the plurality of ligands completely binds with only the first biological target to produce a first electric pulse signature signal and the ligand sensing surface of a second ligand of a plurality of ligands completely binds with only a second biological target to produce a second electric pulse signature signal.

16. The system in accordance with claim 13, further comprising a gel enveloping at least a portion of the plurality of ligands in proximity with the gate region of the FET.

17. The system in accordance with claim 16, further comprising a plurality of ligands, each of the ligands including a ligand sensing surface and a ligand orienting surface opposite the ligand sensing surface, and a plurality of nanotubes provided between a first electrode and a second electrode, wherein the ligand orienting surfaces of the ligands attach to one of the nanotubes.

18. The system in accordance with claim 17, wherein the ligand sensing surface of the first ligand of the plurality of ligands completely binds with only a first biological target to produce a first electrostatic pulse signature signal and the ligand sensing surface of a second ligand of the plurality of ligands completely binds with only a second biological target to produce a second electrostatic pulse signature signal.

19. The system in accordance with claim 17, further comprising a catalyst provided on the gate region.

20. The system in accordance with claim 18, further comprising a catalyst provided on the gate region.

21. The system in accordance with claim 17, further comprising conductive or semi-conductive materials coating the surface of the plurality of nanotubes.

22. The system in accordance with claim 18, further comprising conductive or semi-conductive materials coating the surface of the plurality of nanotubes.

23. The system in accordance with claim 1, further comprising a biological amplification unit connected to the first ligand.

24. The system in accordance with claim 1, wherein the processor records and stores a match between the electric pulse signature signal measured by the measurement means and one of the stored electric pulse signature signals.

25. The system in accordance with claim 1, further comprising a device for collecting energy from the optical spectrum, the energy used to power the sensor unit.

26. The system in accordance with claim 1, further comprising a device for collecting energy from the X-ray and gamma ray spectrum, the energy used to power the sensor unit.

27. The system in accordance with claim 1, further comprising a device for collecting energy from an electromagnetic RF field, the energy used to power the sensor unit.

28. The system in accordance with claim 1, further comprising:

the first ligand of a plurality of ligands, each of the first ligand of the plurality of ligands including a first ligand sensing surface and at least one first ligand orienting surface; and

a second ligand of a plurality of ligands, each of the second ligand of the plurality of ligands including a second ligand sensing surface and at least one second ligand orienting surface, the first and second orienting surfaces of at least a portion of the plurality of ligands provided in proximity to the measurement means.

29. The system according to claim 28 wherein the orienting surface of the first ligand of the plurality of ligands is placed in proximity to the electrostatic sensing surface by a coating applied to the electrostatic sensing surface and a cross-linker that links the orienting surface of the first ligand of the plurality of ligands to the electrostatic sensing surface.

30. The system according to claim 28 wherein the first and second orienting surfaces of the first and second ligand of the plurality of ligands s are placed in proximity to the electrostatic sensing surface by a coating applied to the electrostatic sensing surface and a cross-linker that links the first and second orienting surfaces of the first and second ligand of the plurality of ligands to the electrostatic sensing surface.

31. The system according to claim 28 wherein the orienting surface the first ligand of the plurality of ligands is placed in proximity to the electrostatic sensing surface by using an electrostatic field.

32. The system according to claim 28 wherein the first and second orienting surfaces of the first and second ligand of the plurality of ligands are placed in proximity to the electrostatic sensing surface by using an electrostatic field.

33. The system in accordance with claim 1, further comprising nanotubes utilized to increase a surface area of the electrostatic sensing surface to increase sensing capability for biological target detection.

34. The system in accordance with claim 1 wherein the processor records and stores a match between the first electrostatic pulse signature signal measured by the measurement means and the stored electrostatic pulse signature signals.

35. A system according to claim 1 further comprising:

a) a chemical coating applied to the electrostatic sensing surface; and

b) the first ligand is oriented and tethered to the chemical coating by a cross linker to provide the optimal sensing capability.