Arrays having background features and methods for using the same

• US 7,078,167 B2
• Filed: 07/02/2001
• Issued: 07/18/2006
• Est. Priority Date: 09/17/1999
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Delenstarr, Glenda C., Wolber, Paul K., Sana, Theodore R.
• Primary Examiner(s): Sisson, Bradley L.
• Application Number:
  US09/899,381
• Publication Number:
  US 20020068293A1
• Time in Patent Office: 1,842 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 435/287.2, 435/810, 436/94, 436/800, 436/805, 536/23.1, 536/24.31, 536/24.33
• US Class Current: 435/6.1
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2545/107 with a competitive internal standard/control
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Nucleic acid arrays that have background features, and methods for using the same, are provided. The subject nucleic acid arrays include both hybridization features and background features, where the background features provide a background signal in a hybridization assay that is made up of a feature substrate component, a nucleic acid probe component and a nucleic acid probe non-specific binding component. In practicing the subject methods, the arrays are contacted with a sample and signals are observed for both hybridization features and background features. The background feature signal is then subtracted from the hybridization feature signal to obtain a background corrected hybridization feature signal that is employed as the output of the assay, e.g., to determine the presence, either qualitatively or quantitatively, of the analyte target nucleic acid in the sample. Also provided are kits for use in practicing the subject methods.

Claims

1. A method of detecting the presence of an analyte nucleic acid in a sample, said method comprising:

(a) providing a nucleic acid array comprising;

(i) at least one hybridization feature to which said analyte nucleic acid specifically binds under stringent hybridization conditions; and

(ii) at least one background feature, wherein said background feature is a polymeric composition that comprises background probes that do not hybridize to complementary nucleic acids in said sample;

(b) contacting said nucleic acid array with said sample under stringent hybridization conditions;

(c) washing said nucleic acid array;

(d) detecting a hybridization signal from said hybridization feature and background signal from said background feature;

(e) subtracting said background signal from said hybridization signal to obtain a background corrected hybridization signal; and

(f) relating said background corrected hybridization signal to the presence of said analyte target nucleic acid in said sample to detect the presence of said analyte target nucleic acid in said sample;

wherein said method is further characterized by including a target nucleic acid labeling step prior to said detecting step (d).

2. The method according to claim 1, wherein said labeling step comprises labeling any analyte target nucleic acids present in said sample with a member of a signal producing system prior to said contacting step (b).

3. The method according to claim 1, wherein said labeling step comprises labeling any analyte target nucleic acids present on said array following step(b) with a member of a signal producing system.

4. The method according to claim 1, wherein said background feature provides a background signal following said contacting step that comprises:

(a) a feature substrate background component;

(b) a probe background component; and

(c) a non-specific binding background component.

5. The method according to claim 4, wherein said background probes of said background feature range in length from about 5 to about 100 nt.

6. The method according to claim 4, wherein said background probes are selected from the group consisting of empirically observed inactive probes, probes forming intramolecular structures, short probes, probes comprising reverse polarity nucleotide analogs, probes comprising abasic phosphodiesters or probes comprising modified nucleotidic units.

7. The method according to claim 1, wherein said background feature provides a signal that is the same as a signal generated by a validated background feature made up of empirically observed inactive probes.

8. The method according to claim 7, wherein said validated background feature is made up of nucleic acids having a nucleic acid sequence selected from the group consisting of SEQ ID NOS:

05 to 18, 24 to 32 and 36 to 53.

9. The method according to claim 1, wherein said background feature tests positive in a two-color self-self array hybridization assay.

10. A method of detecting the presence of an analyte nucleic acid in a sample, said method comprising:

(a) providing a nucleic acid array comprising;

(i) at least one hybridization feature to which said analyte nucleic acid specifically binds under stringent hybridization conditions; and

(ii) at least one background feature, wherein said background feature is a polymeric composition that comprises background probes that do not hybridize to complementary nucleic acids in said sample;

(b) contacting said nucleic acid array with said sample under stringent hybridization conditions;

(c) washing said nucleic acid array;

(d) detecting a hybridization signal from said hybridization feature and background signal from said background feature;

(e) subtracting said background signal from said hybridization signal to obtain a background corrected hybridization signal; and

(f) relating said background corrected hybridization signal to the presence of said analyte target nucleic said in said sample to detect the presence of said analyte target nucleic acid in said sample;

wherein said method is further characterized by including a target nucleic acid labeling step prior to said detecting step (d); and

further wherein said background feature provides a background signal following said contacting step that comprises;

(i) a feature substrate background component;

(ii) a probe background component; and

(iii) a non-specific binding background component and tests positive in a two-color self-self array hybridization assay.

11. The method according to claim 10, wherein said labeling step comprises labeling any analyte target nucleic acids present in said sample with a member of a signal producing system prior to said contacting step (b).

12. The method according to claim 10, wherein said labeling step comprises labeling any analyte target nucleic acids present on said array following step(b) with a member of a signal producing system.

13. The method according to claim 12, wherein said background probes of said background feature range in length from about 6 to about 100 nt.

14. The method according to claim 10, wherein said background probes are selected from the group consisting of empirically observed inactive probes, probes forming intramolecular structures, short probes, probes comprising reverse polarity nucleotide analogs, probes comprising abasic phosphodiesters or probes comprising modified nucleotidic units.

15. The method according to claim 10, wherein said background feature provides a signal that is the same as a signal generated by a validated background feature made up of empirically observed inactive probes.

16. The method according to claim 15, wherein said validated background feature is made up of nucleic acids having a nucleic acid sequence selected from the group consisting of SEQ ID NOS:

05 to 18, 24 to 32 and 36 to 53.