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Arrays having background features and methods for using the same

  • US 7,078,167 B2
  • Filed: 07/02/2001
  • Issued: 07/18/2006
  • Est. Priority Date: 09/17/1999
  • Status: Expired due to Term
First Claim
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1. A method of detecting the presence of an analyte nucleic acid in a sample, said method comprising:

  • (a) providing a nucleic acid array comprising;

    (i) at least one hybridization feature to which said analyte nucleic acid specifically binds under stringent hybridization conditions; and

    (ii) at least one background feature, wherein said background feature is a polymeric composition that comprises background probes that do not hybridize to complementary nucleic acids in said sample;

    (b) contacting said nucleic acid array with said sample under stringent hybridization conditions;

    (c) washing said nucleic acid array;

    (d) detecting a hybridization signal from said hybridization feature and background signal from said background feature;

    (e) subtracting said background signal from said hybridization signal to obtain a background corrected hybridization signal; and

    (f) relating said background corrected hybridization signal to the presence of said analyte target nucleic acid in said sample to detect the presence of said analyte target nucleic acid in said sample;

    wherein said method is further characterized by including a target nucleic acid labeling step prior to said detecting step (d).

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