Adipose tissue-derived stromal cell that expresses characteristics of a neuronal cell

• US 7,078,230 B2
• Filed: 02/26/2001
• Issued: 07/18/2006
• Est. Priority Date: 02/26/2000
• Status: Expired due to Fees

• Current Assignee: Vetstem Biopharma, Inc.
• Original Assignee: Artecel Inc.
• Inventors: Wilkison, William O., Gimble, Jeffrey
• Primary Examiner(s): Kemmerer, Elizabeth
• Assistant Examiner(s): Bunner, Bridget E.
• Application Number:
  US09/793,173
• Publication Number:
  US 20010033834A1
• Time in Patent Office: 1,968 Days
• Field of Search: 435/325, 435/352, 435/363, 435/69.1, 435/93.1, 435/93.2, 435/93.7, 424/93.1, 424/93.2, 424/93.7
• US Class Current: 435/325
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C12N 2500/30 Organic components metal chelators C12N2500/10; calcitonin C12N2500/14; transferrin C12N2500/24
• CPC Class Codes: C12N 2500/44 Thiols, e.g. mercaptoethanol
• CPC Class Codes: C12N 2500/80 from animals
• CPC Class Codes: C12N 2506/1384 from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 5/0618 Cells of the nervous system
• CPC Class Codes: C12N 5/0667 Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 5/067 Hepatocytes

Abstract

The invention is in the area of pleuripotent stem cells generated from adipose tissue-derived stromal cells and uses thereof. In particular, the invention includes isolated adipose tissue derived stromal cells that have been induced to express at least one phenotypic characteristic of a neuronal, astroglial, hematopoietic progenitor, or hepatic cell. The invention also includes an isolated adipocyte tissue-derived stromal cell that has been dedifferentiated such that there is an absence of adipocyte phenotypic markers.

Claims

1. An isolated adipose tissue-derived stromal cell that has been differentiated and expresses at least one neuronal marker of a neuronal cell, wherein the neuronal marker is selected from the group consisting of neuron-specific protein (NeuN), neurofilament medium polypeptide (NF-M), neuron-specific enolase (NSE), nestin, trkA, class III beta-tubulin, M subunit of neurofilaments, tyrosine hydroxylase, glutamate receptor subunits of the GluR1–

4 and GluR6 classes, glial fibrillary acidic protein, myelin basic protein, and brain factor 1.

2. An isolated adipose tissue-derived stromal cell that has been differentiated to exhibit at least one characteristic of a neuronal cell, wherein at least one characteristic is a process-like cell extension.