Hybrid yeast-bacteria cloning system and uses thereof
First Claim
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1. A composition comprising:
- a vector for capture, cloning, manipulation, production and delivery of large nucleic acids to a yeast target cell comprising;
(a) a first arm having in sequential order, a first selectable marker, a first cyclization element, and a first segment homologous to the 5′
terminus of a target polynucleotide; and
(b) a second arm having in sequential order, a second selectable marker, a second cyclization element, and a second segment homologous to the 3′
terminus of the target polynucleotide,wherein said vector comprises a polynucleotide sequence homologous to a yeast target polynucleotide sequence for homologous recombination with the target polynucleotide in said yeast target cell, and wherein the target polynucleotide is a polynucleotide of a virus, and said virus is selected from the group consisting of adenovirus, adeno-associated virus, pox virus, papova virus and herpes virus.
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Abstract
A recombinational approach and system for the cloning, manipulation and delivery of large nucleic acids is disclosed. Vectors relying on homologous recombination to mediate the isolation, manipulation and delivery of large nucleic acid segments are disclosed.
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2 Claims
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1. A composition comprising:
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a vector for capture, cloning, manipulation, production and delivery of large nucleic acids to a yeast target cell comprising; (a) a first arm having in sequential order, a first selectable marker, a first cyclization element, and a first segment homologous to the 5′
terminus of a target polynucleotide; and(b) a second arm having in sequential order, a second selectable marker, a second cyclization element, and a second segment homologous to the 3′
terminus of the target polynucleotide,wherein said vector comprises a polynucleotide sequence homologous to a yeast target polynucleotide sequence for homologous recombination with the target polynucleotide in said yeast target cell, and wherein the target polynucleotide is a polynucleotide of a virus, and said virus is selected from the group consisting of adenovirus, adeno-associated virus, pox virus, papova virus and herpes virus. - View Dependent Claims (2)
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Specification
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Current AssigneeCell Genesys Incorporated (ANI Pharmaceuticals, Inc.)
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Original AssigneeCell Genesys Incorporated (ANI Pharmaceuticals, Inc.)
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InventorsMendez, Michael, Finer, Mitchell
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Primary Examiner(s)KETTER, JAMES S
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Application NumberUS09/762,476Time in Patent Office2,246 DaysField of Search435/320.1, 435/483, 435/488, 435/325, 435/91.4, 435/252.1, 536/23.1US Class Current435/252.33CPC Class CodesC12N 15/81 for yeastsC12N 15/86 Viral vectorsC12N 2710/10343 viral genome or elements th...C12N 2800/30 Vector systems comprising s...C12N 2800/70 Vectors containing special ...
