Monkey-origin embryonic stem cells

• US 7,083,977 B2
• Filed: 06/15/2001
• Issued: 08/01/2006
• Est. Priority Date: 06/15/2000
• Status: Expired due to Fees

• Current Assignee: Tanabe Seiyaku Co Ltd (Mitsubishi Chemical Group Corp.)
• Original Assignee: Tanabe Seiyaku Co Ltd (Mitsubishi Chemical Group Corp.)
• Inventors: Torii, Ryuzo, Iritani, Akira, Hosoi, Yoshihiko, Tada, Takashi, Akuta, Teruo, Nakatsuji, Norio
• Primary Examiner(s): Shukla, Ram R.
• Assistant Examiner(s): Montanari, David A.
• Application Number:
  US10/311,146
• Publication Number:
  US 20030157710A1
• Time in Patent Office: 1,873 Days
• Field of Search: 435/6
• US Class Current: 435/325
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 2503/02 Drug screening
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696

Abstract

A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.

Claims

1. A method for producing a Macaca fascicularis embryonic stem cell, comprising:

(a) carrying out fertilization by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and a monkey sperm, thereby giving a fertilized ovum;

(b) allowing the fertilized ovum obtained in the step (a) to differentiate into a blastocyst by in vitro culture at 38°

C., under conditions of at 5% CO₂, 5% O₂ and 90% N₂; and

(c) establishing an embryonic stem cell line using the blastocyst obtained in step (b).

3. The method according to any one of claims 1 to 2, wherein a culture medium selected from the group consisting of TALP solution, TALP-HEPES solution and BWW solution is used in step (a).

4. The method according to any one of claims 1 to 2, wherein the in vitro culture in step (b) is a micro-hanging drop culture.

5. The method according to any of claims 1 to 2, wherein the in vitro culture comprising said blastocyst is performed using CMRL-1066.

2. A method for producing a Macaca fuscata embryonic stem cell comprising:

(a) carrying out fertilization by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and a monkey sperm, thereby giving a fertilized ovum;

(b) allowing the fertilized ovum obtained in step (a) to differentiate into a blastocyst by in vitro culture at 38°

C., under conditions at 5% CO₂, 5% O₂ and 90% N₂; and

(c) establishing an embryonic stem cell line using the blastocyst obtained in step (b).