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Tn5 transposase mutants and the use thereof

  • US 7,083,980 B2
  • Filed: 04/16/2004
  • Issued: 08/01/2006
  • Est. Priority Date: 04/17/2003
  • Status: Expired due to Term
First Claim
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1. A polypeptide comprising a Tn5 transposase mutant modified relative to SEQ ID NO:

  • 2, the transposase mutant comprising a mutation at position 54 of SEQ ID NO;

    2, a mutation at position 242of SEQ ID NO;

    2, and a mutation at position 372of SEQ ID NO;

    2, wherein the transposase mutant has greater avidity than wild-type Tn5 transposase for at least one of a Tn5 outside end sequence as defined by SEQ ID NO;

    3 and a modified Tn5 outside end sequence as defined by SEQ ID NO;

    5.

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