Animal cage behavior system

• US 7,086,350 B2
• Filed: 07/25/2003
• Issued: 08/08/2006
• Est. Priority Date: 07/25/2002
• Status: Expired due to Fees

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Tecott, Laurence H., Goulding, Evan
• Primary Examiner(s): Swiatek, Robert P.
• Application Number:
  US10/627,187
• Publication Number:
  US 20050066910A1
• Time in Patent Office: 1,110 Days
• Field of Search: 119/51.02, 119/417, 119/420, 119/421
• US Class Current: 119/421
• CPC Class Codes: A01K 1/031 Cages for laboratory animals; Cages for measuring metabolism of animals
• CPC Class Codes: A01K 29/005 Monitoring or measuring activity, e.g. detecting heat or mating

Abstract

A behavioral monitoring system useful for the analysis of complex behaviors in a number of animal species. The monitoring systems of this invention allow continuous monitoring of feeding, drinking and movement of animals with high temporal and spatial resolution. In certain embodiments, the system comprises an enclosure comprising: an animal position indicator; a food consumption indicator; and a fluid consumption indicator, where the system reports behavioral data at a temporal resolution of 20 seconds or lower.

Claims

1. An animal behavior analysis system, said system comprising:

an enclosure, said enclosure comprising;

an animal position indicator comprising a two-dimensional force plate;

a food consumption indicator comprising a photobeam and an overflow; and

a fluid consumption indicator comprising a capacitance indicator;

wherein said system reports behavioral data at a temporal resolution of 20 seconds or lower.

2. The system of claim 1, wherein said system reports behavioral data with a temporal resolution of 1 second or lower.

3. The system of claim 1, wherein said system reports behavioral data with a temporal resolution of 50 ms or lower.

4. The system of claim 1, wherein said enclosure is a mouse cage.

5. The system of claim 1, wherein said system reports behavioral data with a temporal resolution sufficient to permit automated bout detection of a mouse.

6. The system of claim 1, wherein said force plate comprises high and low frequency transducers.

7. The system of claim 1, wherein said food consumption indicator comprises an overflow to prevent piled up food from producing a false positive signal.

8. The system of claim 1, wherein said capacitance detector operates in a radio frequency range.

9. The system of claim 1, further comprising a data acquisition system.

10. The system of claim 9, wherein said data acquisition system produces an event file.

11. The system of claim 10, wherein the event file produced by said system provides event information at sufficient resolution to distinguish behavioral bouts of mice using a mathematical method.

12. The system of claim 11, wherein said mathematical method comprises a frequency analysis.

13. A method of characterizing the behavior of an animal, said method comprising:

providing an animal in an animal behavior analysis system of claim 1; and

collecting data produced by said system wherein data provided by said system comprises event information regarding spatial position of the animal in the enclosure, event information regarding food consumption, and event information regarding fluid consumption.

14. The method of claim 13, wherein said animal is a mouse.

15. The method of claim 13, wherein said method further comprises analyzing said data to provide a behavioral fingerprint of said animal.

16. The method of claim 15, wherein said analyzing comprises automated detection of one or more behavioral bouts.

17. The method of claim 15, wherein said behavioral fingerprint is characteristic for a particular genetic strain of said animal.

18. The method of claim 15, further comprising entering said fingerprint into a database of behavioral fingerprints.

19. The method of claim 13, wherein said data is provided in an event file.

20. The method of claim 13, further comprising taking a moving average of said event data.

21. The method of claim 13, further comprising performing a frequency analysis of said event data.

22. A method of screening an agent for an effect on animal behavior, said method comprising:

contacting a test animal with said agent;

placing said animal in an animal behavior analysis system of claim 1 to generate a behavioral fingerprint;

and comparing said fingerprint to a negative control fingerprint where a deviation of said fingerprint from said negative fingerprint control indicates that said agent alters said animal'"'"'s behavior.

23. The method of claim 22, wherein said agent is a substance of abuse.

24. The method of claim 22, wherein said agent is a pharmaceutical.

25. The method of claim 22, wherein said animal is a mouse.

26. The method of claim 22, wherein said animal is a mutant mouse.