Binding acceleration techniques for the detection of analytes

• US 7,087,148 B1
• Filed: 11/13/2000
• Issued: 08/08/2006
• Est. Priority Date: 06/23/1998
• Status: Expired due to Term

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Vielmetter, Jost G., Kayyem, Jon Faiz, Blackburn, Gary
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US09/712,792
• Time in Patent Office: 2,094 Days
• Field of Search: 204/403.01, 204/403.03, 204/403.04, 204/403.05, 204/403.06, 204/403.07, 204/409, 204/411, 204/412, 204/603, 204/452, 204/461, 204/612
• US Class Current: 205/452
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: G01N 27/44704 Details; Accessories

Abstract

The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the target analyte, either directly or indirectly, to allow electronic detection of the ETM.

Claims

1. A device comprising a substrate comprising:

a) a first surface comprising;

i) an array of detection electrodes each comprising a covalently attached capture ligand;

ii) at least a first electrophoresis electrode;

b) a second surface comprising at least a second electrophoresis electrode; and

c) at least one channel connecting said first surface and second surface.

2. A device according to claim 1 wherein said channel comprises a permeation layer material.

3. A device according to claim 2 wherein said channel comprises a membrane.

4. A device according to claim 1 further comprising a detection chamber, wherein said detection chamber comprises said surfaces.

5. A device according to claim 1 wherein said detection electrodes further comprise a self-assembled monolayer.

6. A device according to claim 1 wherein said substrate is Printed Circuit (PC) board.

7. A device according to claim 1 wherein said capture ligand is nucleic acid.

8. A device comprising a detection chamber comprising:

a) a first subchamber comprising an array of detection electrodes each comprising a covalently attached capture ligand;

b) a second subchamber comprising an absorbent material; and

c) a substrate comprising;

i) a first surface in contact with said array of detection electrodes;

ii) a second surface in contact with said absorbent material;

iii) at least one channel connecting said first surface and said second surface; and

iv) a size exclusion partition at either end of said at least one channel.

9. A device according to claim 8 wherein said channel comprises a permeation layer material.

10. A device according to claim 8 wherein said channel comprises a membrane.

11. A device according to claim 8 wherein said detection electrodes further comprise a self-assembled monolayer.

12. A device according to claim 8 wherein said substrate is PC board.

13. A device according to claim 8 wherein said capture ligand is nucleic acid.

14. The device according to claim 8, wherein said size exclusion partition is in contact with said first surface.

15. The device according to claim 8, wherein said size exclusion partition is in contact with said second surface.

16. The device according to claim 8, wherein said size exclusion partition is a semi-permeable membrane.

17. A method of detecting a target analyte in a sample comprising:

a) concentrating said target analyte in a detection chamber comprising;

i) a first surface comprising;

1) an array of detection electrodes each comprising a covalently attached capture ligand;

2) at least a first electrophoresis electrode;

ii) a second surface comprising at least a second electrophoresis electrode; and

iii) at least one channel connecting said first surface and second surface;

b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and

c) detecting the presence of said ETM using said detection electrode.

18. A method according to claim 17 wherein said detection electrodes further comprise a self-assembled monolayer.

19. A method according to claim 17 wherein said detection chamber further comprises a substrate.

20. A method according to claim 19 wherein said substrate is a PC board.

21. A method according to claim 17 wherein said capture ligand is nucleic acid.

22. A method according to claim 17 wherein said assay complex comprises said target analyte, said capture ligand, and a label probe and wherein said label probe comprises said ETM.

23. A method according to claim 17 wherein said assay complex comprises said target analyte and said capture ligand and said target analyte comprises said ETM.

24. A method according to claim 17 wherein said ETM is a transition metal complex.

25. A method of detecting a target analyte in a sample comprising:

a) concentrating said target analyte in a detection chamber comprising;

i) a substrate comprising;

1) a first surface comprising;

A) an array of detection electrodes each comprising a covalently attached capture ligand;

2) a second surface;

3) at least one channel connecting said first surface and second surface; and

ii) an absorbent material in contact with said second surface;

b) binding said target analyte to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and

c) detecting the presence of said ETM using said detection electrode.

26. A method according to claim 25 wherein said detection electrodes further comprise a self-assembled monolayer.

27. A method according to claim 25 wherein said substrate is PC board.

28. A method according to claim 25 wherein said capture ligand is nucleic acid.

29. A method according to claim 25 wherein said assay complex comprises said target analyte, said capture ligand, and a label probe and wherein said label probe comprises said ETM.

30. A method according to claim 25 wherein said assay complex comprises said target analyte and said capture ligand and said target analyte comprises said ETM.

31. A method according to claim 25 wherein said ETM is a transition metal complex.

32. The method according to claim 25 wherein said detection chamber further comprises a size exclusion partition at either end of said at least one channel.