Nucleic acid archiving

• US 7,087,387 B2
• Filed: 10/21/2003
• Issued: 08/08/2006
• Est. Priority Date: 04/16/1997
• Status: Expired due to Term

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Marmaro, Jeffery M., Roehl, Christopher A., Ives, Jeffrey T., Gerdes, John C.
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US10/690,359
• Publication Number:
  US 20040091925A1
• Time in Patent Office: 1,022 Days
• Field of Search: 435/6, 536/23.1, 536/24.3
• US Class Current: 435/6.12
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C12N 15/1006 by means of a solid support carrier, e.g. particles, polymers
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer
• CPC Class Codes: C12Q 2531/113 PCR
• CPC Class Codes: C12Q 2537/143 Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target

Abstract

This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method for virus extraction, purification, and solid phase amplification from large volume plasma specimens is described.

Claims

1. A kit for nucleic acid manipulation, comprising:

(a) a substrate having a surface coated with a solid phase matrix, wherein said matrix is a specific binding material having one or more electropositive materials rendered hydrophilic; and

(b) one or more containers comprising buffers and/or reagents necessary for manipulating said nucleic acid.

2. The kit of claim 1, wherein said substrate is in the shape of tubes, plates, membranes, capillaries, slides beads, microparticles, fibers, microchannels, and microarrays.

3. The kit of claim 1, wherein said substrate is a polymer or an oxide substrate.

4. The kit of claim 1, wherein said electropositive material comprises one or more elements selected from the group consisting of aluminum, titanium, zirconium, hafnium, scandium, yttrium, lanthanum, vanadium, tantalum, chromium, molybdenum, tungsten, boron, gallium, indium, germanium, tin, and lead.

5. The kit of claim 1, wherein said matrix is selected from the group consisting of aluminum oxide, titanium oxide (Ti₂O₃), and modified zirconium dioxide (ZrO₂).

6. The kit of claim 1, wherein said matrix is selected from the group consisting of alpha aluminum oxide, gamma aluminum oxide and an aluminum oxide thin-film of mixed composition.

7. The kit of claim 1, wherein said solid phase matrix is titanium oxide (Ti₂O₃).

8. The kit of claim 1, wherein said solid phase matrix is modified zirconium oxide (ZrO₂).

9. The kit of claim 1, wherein said reagents include reagents for amplifying said nucleic acid.