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Vitamin D assay

  • US 7,087,395 B1
  • Filed: 01/16/2001
  • Issued: 08/08/2006
  • Est. Priority Date: 01/16/2001
  • Status: Expired due to Term
First Claim
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1. A kit for determining a concentration of 25-hydroxy vitamin D (25-OH-D) in a sample, comprising:

  • a releasing composition including from about 0.01 to about 5% of a cyclodextrin, from about 0.01 to about 5% of a sodium salicylate, and from about 0.1 to about 1.0 M NaOH, the cyclodextrin, the salicylate, and the NaOH being provided in an amount effective to reduce interference from a protein or a lipid with 25-hydroxy vitamin D present in a sample;

    25-hydroxy vitamin D coupled to a solid phase;

    vitamin D binding protein; and

    a vitamin D binding protein antibody coupled to a label present in an amount that produces a detectable signal when 25-hydroxy vitamin D is present in the sample.

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