Fluorescent nucleotides and labeling methods using the same
First Claim
1. A fluorescent nucleotide represented by the formula:
- X—
Y—
Z wherein,X represents a residue of a natural or non-natural nucleotide, an oligonucleotide, or a polynucleotide, which binds to Y at a basic moiety of said residue;
Y represents a divalent bridging group or a single bond; and
Z represents a monovalent group derived from;
a compound represented by the following general formula (II);
2 Assignments
0 Petitions
Accused Products
Abstract
Fluorescent nucleotides useful for labeling nucleic acids which are represented by the formula: X—Y—Z wherein X represents a residue of natural or non-natural nucleotide and the like and binds to Y at a basic moiety of the residue; Y represents a divalent bridging group or a single bond; Z represents a monovalent group derived from a compound represented by the formula (I) wherein R1 to R4 represent an alkyl group; V1 to V6 represent a hydrogen atom or a substituent; L1 to L7 represent a methine group; W represents an oxygen atom, a sulfur atom, —C(R3)(R4)— or —N(R5)— wherein R3 to R5 represents an alkyl group; Q represents a nitrogen atom or —C(V7)— wherein V7 represents a hydrogen atom or a monovalent substituent; M represents a counter ion; m represents a number required to neutralize the charge of the molecule; s, t and u represent 0 or 1 or the like, and binds to Y at a reactive group existing in R1 or R2
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Citations
15 Claims
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1. A fluorescent nucleotide represented by the formula:
- X—
Y—
Z wherein,X represents a residue of a natural or non-natural nucleotide, an oligonucleotide, or a polynucleotide, which binds to Y at a basic moiety of said residue; Y represents a divalent bridging group or a single bond; and Z represents a monovalent group derived from; a compound represented by the following general formula (II); - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- X—
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14. An azamethine dye represented by the following general formula (II):
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15. An azamethine dye represented by the following general formula (III):
Specification