Focused acoustic energy in the preparation of peptide arrays
First Claim
1. A method for preparing an array comprised of a plurality of peptidic molecules attached to a substrate surface, the method comprising applying focused acoustic energy to each of a plurality of reservoirs each containing a peptidic molecule in a fluid, wherein the distance between the centers of any two adjacent reservoirs is less than about 1 centimeter, and the focused acoustic energy is applied in a manner effective to eject a droplet from each reservoir toward a different site on a substrate surface, wherein the peptidic molecules are selected from the group consisting of pharmacologically active peptides, polypeptides, and proteins.
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Abstract
The present invention relates to arrays of peptidic molecules and the preparation of peptide arrays using focused acoustic energy. The arrays are prepared by acoustically ejecting peptide-containing fluid droplets from individual reservoirs towards designated sites on a substrate for attachment thereto.
66 Citations
35 Claims
- 1. A method for preparing an array comprised of a plurality of peptidic molecules attached to a substrate surface, the method comprising applying focused acoustic energy to each of a plurality of reservoirs each containing a peptidic molecule in a fluid, wherein the distance between the centers of any two adjacent reservoirs is less than about 1 centimeter, and the focused acoustic energy is applied in a manner effective to eject a droplet from each reservoir toward a different site on a substrate surface, wherein the peptidic molecules are selected from the group consisting of pharmacologically active peptides, polypeptides, and proteins.
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19. A method for preparing a peptide array comprised of a plurality of peptidic molecules attached to a substrate surface, the method comprising:
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(a) acoustically coupling a first reservoir containing a first peptidic molecule in a first fluid to an ejector that produces acoustic radiation; (b) activating the ejector to generate acoustic radiation having a focal point sufficiently near the surface of the first fluid so as to eject a droplet thereof toward a first site on the substrate surface; (c) acoustically coupling a second reservoir containing a second peptidic molecule in a second fluid to the ejector; (d) activating the ejector as in step (b) to eject a droplet of the second fluid from the second reservoir toward a second site on the substrate surface; and (e) repeating steps (c) and (d) with additional reservoirs each containing a peptidic molecule in a fluid until a droplet has been ejected from each reservoir; wherein the peptidic molecules are selected from the group consisting of pharmacologically active peptides, polypeptides, and proteins. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification