Amplification of nucleic acids with electronic detection
First Claim
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1. A kit for the detection of an amplification reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising:
- a) a first nucleic acid primer substantially complementary to at least a first domain of said first target sequence;
b) a first label probe substantially complementary to at least a second domain of said first target sequence, said first label probe comprising a plurality of electron transfer moieties (ETM);
c) a second nucleic acid primer substantially complementary to at least a first domain of said second target sequence;
d) a second label probe substantially complementary to at least a second domain of said second target sequence, said second label probe comprising a plurality of electron transfer moieties (ETM); and
, e) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence.
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Abstract
The invention relates to compositions and methods useful in the detection of nucleic acids using a variety of amplification techniques, including both signal amplification and target amplification. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the nucleic acid, either directly or indirectly, to allow electronic detection of the ETM using an electrode.
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Citations
18 Claims
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1. A kit for the detection of an amplification reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising:
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a) a first nucleic acid primer substantially complementary to at least a first domain of said first target sequence;
b) a first label probe substantially complementary to at least a second domain of said first target sequence, said first label probe comprising a plurality of electron transfer moieties (ETM);
c) a second nucleic acid primer substantially complementary to at least a first domain of said second target sequence;
d) a second label probe substantially complementary to at least a second domain of said second target sequence, said second label probe comprising a plurality of electron transfer moieties (ETM); and
,e) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence. - View Dependent Claims (3, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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2. A kit for the detection of a PCR amplification reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising:
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a) a first pair of PCR primers for the amplification of said first target sequence;
b) a first label probe which is substantially complementary to at least a first domain of said first target sequence, wherein said label probe comprises a plurality of electron transfer moieties (ETM);
c) a second pair of PCR primers for the amplification of said second target sequence;
d) a second label probe which is substantially complementary to at least a first domain of said second target sequence, wherein said label probe comprises a plurality of electron transfer moieties (ETM); and
,e) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence. - View Dependent Claims (7)
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4. A kit for the detection of a LCR amplification reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising:
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a) a first nucleic acid primer substantially complementary to at least a first domain of said first target sequence;
b) a second nucleic acid primer substantially complementary to at least a first domain of said second target sequence;
c) a first label probe substantially complementary to a first domain of said first nucleic acid primer, wherein said label probe comprises a plurality of electron transfer moieties (ETM);
d) a second label probe substantially complementary to a first domain of said second nucleic acid primer, wherein said label probe comprises a plurality of electron transfer moieties (ETM);
e) a ligase; and
,f) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence.
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5. A kit for the detection of a strand displacement amplification (SDA) reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising:
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a) a first nucleic acid primer substantially complementary to at least a first domain of said first target sequence;
b) a second nucleic acid primer substantially complementary to at least a first domain of said second target sequence;
c) a polymerase that will modify said first and second nucleic acid primers;
d) a nicking enzyme;
e) a first label probe substantially complementary to a first domain of said first nucleic acid primer, wherein said label probe comprises a plurality of electron transfer moieties (ETM);
f) a second label probe substantially complementary to a first domain of said second nucleic acid primer, wherein said label probe comprises a plurality of electron transfer moieties (ETM); and
,g) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence.
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6. A kit for the detection of a NASBA amplification reaction of a plurality of target nucleic acid sequences comprising at least a first and a second target sequence, wherein said first and second target sequences are different, said kit comprising:
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a) a first nucleic acid primer substantially complementary to at least a first domain of said first target sequence;
b) a second nucleic acid primer substantially complementary to at least a first domain of said second target sequence;
c) a reverse transcriptase, d) a RNA degrading enzyme, e) a DNA polymerase;
f) a RNA polymerase;
g) a first label probe substantially complementary to a first domain of said first nucleic acid primer, wherein said label probe comprises a plurality of electron transfer moieties (ETM);
h) a second label probe substantially complementary to a first domain of said second nucleic acid primer, wherein said label probe comprises a plurality of electron transfer moieties (ETM); and
,i) a substrate comprising at least a first and a second electrode, wherein said first electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a first capture probe substantially complementary to a domain of said first target sequence, and wherein said second electrode comprises a self-assembled mixed monolayer (SAM) comprising a first SAM-forming species and a second SAM-forming species comprising a second capture probe substantially complementary to a domain of said second target sequence.
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Specification