DNA molecules encoding BMP receptor proteins

• US 7,091,007 B2
• Filed: 06/23/2003
• Issued: 08/15/2006
• Est. Priority Date: 09/17/1993
• Status: Expired due to Fees

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Thies, R. Scott, Yamaji, Noboru, Wozney, John M., Celeste, Anthony J.
• Primary Examiner(s): Li, Ruixiang
• Application Number:
  US10/600,645
• Publication Number:
  US 20040142417A1
• Time in Patent Office: 1,149 Days
• Field of Search: 435/69.1, 435/320.1, 435/325, 530/350
• US Class Current: 435/69.7
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C07K 14/71 for growth factors; for growth regulators

Abstract

Novel serine/threonine receptor proteins and BMP receptor proteins are disclosed, as well as DNA molecules encoding the BMP receptor proteins and methods of using the receptor proteins. Further disclosed are truncated BMP receptor proteins and molecules which act as ligands to the BMP receptor proteins.

Claims

1. An isolated DNA molecule comprising a DNA sequence encoding a BMP receptor protein, wherein the DNA sequence comprises nucleotides 61 to 1656 of SEQ ID NO:

1.

3. An isolated host cell transformed with the DNA molecule of claim 1.

2. An isolated DNA molecule comprising a DNA sequence encoding a BMP receptor protein, wherein the DNA sequence comprises nucleotides encoding for amino acids 24 to 532 of SEQ ID NO:

2.

4. An isolated host cell transformed with the DNA molecule of claim 2.

5. An isolated DNA molecule having a sequence encoding a truncated BMP receptor protein which is characterized by the ability to bind to BMP-2 or BMP-4 in a binding assay, said DNA molecule comprising a DNA sequence selected from the group consisting of:

(a) nucleotide 61 to 507 of SEQ ID NO;

1;

(b) nucleotides encoding amino acids 1 to 149 of SEQ ID NO;

2; and

(c) nucleotides encoding amino acids 24 to 149 of SEQ ID NO;

2.

6. An isolated host cell transformed with the DNA molecule of claim 5.

7. A vector comprising a DNA molecule of claim 5 in operative association with an expression control sequence therefor.

8. A method for producing a purified truncated BMP receptor protein, said method comprising the steps of:

(a) culturing in a culture medium a host cell transformed with a DNA sequence according to claim 5, comprising a nucleotide sequence encoding a truncated BMP receptor protein; and

(b) recovering and purifying said truncated BMP receptor protein from the culture medium.

9. A method for producing a truncated BMP receptor protein, said method comprising the steps of:

(a) culturing in a culture medium a host cell according to claim 6, comprising a truncated nucleotide sequence encoding the ligand binding domain of a BMP receptor protein; and

(b) recovering and purifying said BMP receptor protein from the culture medium.

10. An isolated DNA molecule comprising the DNA sequence of CFK1-23a deposited under ATCC accession number 69378.