Enhanced diffraction-based biosensor devices
First Claim
1. A method of detecting an analyte of interest in a medium with a diffraction based biosensor having at least one additional detection tag mechanism, comprising:
- exposing a detection tag material to the medium, the detection tag material tagging the analyte of interest with a measurable parameter in addition to optical diffraction characteristics of the analyte of interest;
exposing a biosensor to the medium, the biosensor having a substrate member with a receptive material layer applied to at least one side thereof wherein the receptive material is specific for the analyte of interest such that the analyte binds to the receptive material;
separating an unbound detection tag material from a bound detection tag material tagging the analyte of interest;
exposing the biosensor to a light source and detecting the presence of the analyte by detecting a diffraction pattern of the transmitted light or reflected light; and
detecting the emitted measurable parameter of the detection tag material.
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Accused Products
Abstract
An enhanced diffraction based biosensor system and method are provided for detecting an analyte of interest in a test medium. The system incorporates at least one additional detection tag substance with the analyte of interest, the tag emitting a measurable parameter that is different from optical diffraction characteristics of the analyte. The biosensor may be a “fluoroptical” system wherein the detection tag is a fluorescence emitting substance, including fluorescent-labeled diffraction enhancing elements. The enhanced diffraction biosensor system may determine the presence of analytes in biological fluids both qualitatively and quantitatively.
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Citations
21 Claims
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1. A method of detecting an analyte of interest in a medium with a diffraction based biosensor having at least one additional detection tag mechanism, comprising:
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exposing a detection tag material to the medium, the detection tag material tagging the analyte of interest with a measurable parameter in addition to optical diffraction characteristics of the analyte of interest; exposing a biosensor to the medium, the biosensor having a substrate member with a receptive material layer applied to at least one side thereof wherein the receptive material is specific for the analyte of interest such that the analyte binds to the receptive material; separating an unbound detection tag material from a bound detection tag material tagging the analyte of interest; exposing the biosensor to a light source and detecting the presence of the analyte by detecting a diffraction pattern of the transmitted light or reflected light; and detecting the emitted measurable parameter of the detection tag material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification