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Purification of substances by reaction affinity chromatography

  • US 7,094,351 B2
  • Filed: 10/12/2001
  • Issued: 08/22/2006
  • Est. Priority Date: 10/12/2000
  • Status: Expired due to Fees
First Claim
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1. A method of separating a target from a sample composition containing the target, comprising the following steps:

  • (a) contacting the sample composition with a reactive affinity molecule attached to a phase separating group, the reactive affinity molecule comprising a reactive functional group, and the reactive affinity molecule reacting with the target to farm an adduct by forming a covalent bond between the target and the reactive functional group, where the reaction forming the adduct is reversible under the conditions of the contacting without the addition of a reagent acting at the covalent bond, to reform the target and reactive affinity molecule(b) separating the adduct from the sample composition;

    (c) contacting the adduct with an eluent to regenerate the target and the reactive affinity molecule; and

    (d) separating the target from the reactive affinity molecule.

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