Support for a method for determining an analyte and a method for producing the support

• US 7,097,974 B1
• Filed: 08/27/1999
• Issued: 08/29/2006
• Est. Priority Date: 08/28/1998
• Status: Expired due to Fees

• Current Assignee: Comprehensive Biomarker Center GmbH
• Original Assignee: Febit Biotech GmbH
• Inventors: Müller, Manfred, Lindner, Hans, Stähler, Peer F., Stähler, Cord F., Stähler, Fritz
• Primary Examiner(s): Ponnaluri, Padmashri
• Assistant Examiner(s): TRAN, MY CHAU T
• Application Number:
  US09/763,914
• Time in Patent Office: 2,559 Days
• Field of Search: 435/6, 435/970, 435/973, 436/89, 436/518, 436/527, 436/528, 536/25.1, 536/25.3, 536/25.31
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/00436 Maskless processes
• CPC Class Codes: B01J 2219/00439 using micromirror arrays
• CPC Class Codes: B01J 2219/00441 using lasers
• CPC Class Codes: B01J 2219/00448 using microlens arrays
• CPC Class Codes: B01J 2219/00479 Means for mixing reactants or products in the reaction vessels
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00511 Walls of reactor vessels
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00603 Making arrays on substantially continuous surfaces
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 : by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00637 : by coating it with another layer
• CPC Class Codes: B01J 2219/00648 : by the use of solid beads
• CPC Class Codes: B01J 2219/00659 : Two-dimensional arrays
• CPC Class Codes: B01J 2219/00675 : In-situ synthesis on the substrate
• CPC Class Codes: B01J 2219/00689 : using computers
• CPC Class Codes: B01J 2219/00702 : Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00704 : integrated with the reactor apparatus
• CPC Class Codes: B01J 2219/00711 : Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01L 2300/0654 : Lenses; Optical fibres
• CPC Class Codes: B01L 2300/069 : Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0816 : Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 : comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12N 15/10 : Processes for the isolation, preparation or purification of DNA or RNA chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34
• CPC Class Codes: C12P 19/34 : Polynucleotides, e.g. nucleic acids, oligoribonucleotides
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 50/14 : Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
• CPC Class Codes: G01N 21/253 : for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/6454 : using an integrated detector array
• CPC Class Codes: G01N 33/551 : the carrier being inorganic
• CPC Class Codes: G03F 7/70216 : Mask projection systems
• CPC Class Codes: Y10S 435/97 : Test strip or test slide
• CPC Class Codes: Y10S 435/973 : Simultaneous determination of more than one analyte

Abstract

A method for producing a support for determining analytes. The method comprises the steps of (a) providing a support comprising at least one channel, comprising a conduit having an intake and an outlet for passing fluid from the intake to the outlet, in the support body, (b) passing liquid with building blocks for synthesizing polymeric receptors through the channel or channels of the support body, (c) site- and/or time-specifically immobilizing the receptor building blocks in each case on predetermined positions in the channel or channels by illumination and (d) repeating steps (b) and (c) until the required receptors have been synthesized in each case on the predetermined positions.

Claims

1. A method for producing a support containing polymeric receptors for determining analytes, comprising the steps of

(a) providing a support, wherein said support comprises a support body comprising at least one channel which comprises a conduit with a top, a bottom and two sides having an inlet and an outlet for passing fluid from the inlet to the outlet, and at least one reaction position in the support body, wherein said support is optically transparent at least in the region of said at least one reaction position,

(b) placing said support body between a programmable light source matrix and a detector matrix,

(c) passing liquid with building blocks for synthesizing said polymeric receptors through the channel or channels of the support body,

(d) site- and/or time-specifically immobilizing the said building blocks on predetermined reaction positions in the channel or channels by illumination of said predetermined reaction positions by said programmable light source matrix, wherein the illumination position of said light source matrix is monitored by computer using said detector matrix, and

(e) repeating steps (c) and (d) until said polymeric receptors have been synthesized on the predetermined positions.

2. The method as claimed in claim 1, wherein said method produces a support which comprises defined areas, each of which contains identical receptor species.

3. The method as claimed in claim 1 wherein said channels are arranged on the surface of said support.

4. The method as claimed in claim 1 wherein said support comprises several hundred channels per support body and said channels are arranged parallel to one another.

5. The method as claimed in claim 1 wherein said polymeric receptors are selected from the group consisting of nucleic acids and nucleic acid analogs.

6. The method as claimed in claim 5, wherein said building blocks are selected from the group consisting of nucleotides, oligonucleotides, nucleotide analogs and oligonucleotide analogs.

7. The method as claimed in claim 1 characterized in that the illumination takes place via a programmable light source matrix.

8. The method as claimed in claim 1 characterized in that the pattern of polymeric receptors is determined by computer programming.

9. The method as claimed in claim 1, wherein said at least one channel is a capillary channel.

10. The method as claimed in claim 1, wherein said at least one channel contains a plurality of said polymeric receptors.

11. The method as claimed in claim 1, wherein said at least one channel provides a three dimensional surface area for synthesis of said polymeric receptors.